Scholarlink

<p>Tiller number is an essential agronomic characteristic that influences barley morphology and yield. A barley low number of tillers mutant CIHO 11,530 exhibits few tillers and in this study, we conducted a genetic analysis of the barley <i>low number of tillers 2</i> (<i>lnt2</i>) locus. Linkage analysis showed that <i>lnt2</i> was mapped in an interval of 3.39&#xa0;cM on chromosome 6HS between the flanking markers <i>SNP1765</i> and <i>SNP526</i>, explaining 53.06% of the phenotypic variance. The genetic effect of <i>lnt2</i> was further verified in two other genetic backgrounds, explaining variances of 86.43% and 91.01% in tiller numbers between lines carrying the <i>lnt2</i> mutant and wild-type alleles, respectively. Furthermore, we constructed a large F<sub>2</sub> population and fine-mapped <i>lnt2</i>. Finally, <i>lnt2</i> was mapped within a 0.19&#xa0;cM genetic interval delimited by the tightly linked KASP markers <i>KASP6359</i> and <i>KASP365</i>, and the physical interval was located at 40.57–42.35&#xa0;Mb. In this interval, three genes were highly likely <i>lnt2</i> based on gene annotations, sequence and gene expression analyses. Our research provides valuable information for the map-based cloning of <i>lnt2</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fine-mapping the low number of tillers (lnt2) locus in barley

  • 期刊论文

  • 发表日期：

    2025年9月9日

查看全文
  • Shuai Hou,
  • Hong Zhou,
  • Jinxiong Lv,
  • Peng Chen,
  • Caixia Li,
  • Yu Lin,
  • Yueyue Liu,
  • Yaxi Liu

摘要

Tiller number is an essential agronomic characteristic that influences barley morphology and yield. A barley low number of tillers mutant CIHO 11,530 exhibits few tillers and in this study, we conducted a genetic analysis of the barley low number of tillers 2 (lnt2) locus. Linkage analysis showed that lnt2 was mapped in an interval of 3.39 cM on chromosome 6HS between the flanking markers SNP1765 and SNP526, explaining 53.06% of the phenotypic variance. The genetic effect of lnt2 was further verified in two other genetic backgrounds, explaining variances of 86.43% and 91.01% in tiller numbers between lines carrying the lnt2 mutant and wild-type alleles, respectively. Furthermore, we constructed a large F2 population and fine-mapped lnt2. Finally, lnt2 was mapped within a 0.19 cM genetic interval delimited by the tightly linked KASP markers KASP6359 and KASP365, and the physical interval was located at 40.57–42.35 Mb. In this interval, three genes were highly likely lnt2 based on gene annotations, sequence and gene expression analyses. Our research provides valuable information for the map-based cloning of lnt2.