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<p>Eucommiae Folium (EF) is a dual-purpose substance utilized in both medicine and nutrition, commonly incorporated into porridge and tea. Traditionally, it is recognized for its ability to tonify the kidney and liver, strengthen muscles and bones, lower blood pressure, and exhibit anti-aging effects. This study aims to investigate the primary components and mechanisms underlying the anti-rheumatoid arthritis (RA) properties of aqueous extract of EF. To identify the material basis of aqueous extract of EF, we employed liquid chromatography-mass spectrometry (LC-Orbitrap-MS) for component analysis using database software. Subsequently, a network approach was applied to pinpoint key anti-RA targets and pathways in order to validate its potential as an effective anti-RA agent. An adjuvant-induced arthritis (AIA) rat model was established, with oral administration of aqueous extract of EF over 30 consecutive days to evaluate its therapeutic effects. The results indicated that LC-Orbitrap-MS identified 29 principal compounds, including geniposidic acid, asperuloside, chlorogenic acid, kaempferol, aucubin, and trifolin. Pharmacological network analysis suggested that these components may exert their therapeutic effects on RA through modulation of NF-κB and PI3K-AKT signaling pathways. Furthermore, oral administration significantly reduced paw swelling in AIA rats while histological analysis demonstrated suppression of inflammatory responses in paw tissues by inhibiting NF-κB and PI3K-AKT pathways.</p>

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Integrated LC-Orbitrap-MS and network pharmacology decipher the pharmacological basis of Eucommiae folium in treating rheumatoid arthritis

  • 期刊论文

  • 发表日期：

    2026年3月4日

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  • Yasi Deng,
  • Ling Liang,
  • Haokai Lin,
  • Xinyang Shen,
  • Hao Zheng,
  • Ying Deng,
  • Xing Tian,
  • Juan Huang,
  • Ye Zhang,
  • Bin Li,
  • Huanghe Yu,
  • Wei Wang

摘要

Eucommiae Folium (EF) is a dual-purpose substance utilized in both medicine and nutrition, commonly incorporated into porridge and tea. Traditionally, it is recognized for its ability to tonify the kidney and liver, strengthen muscles and bones, lower blood pressure, and exhibit anti-aging effects. This study aims to investigate the primary components and mechanisms underlying the anti-rheumatoid arthritis (RA) properties of aqueous extract of EF. To identify the material basis of aqueous extract of EF, we employed liquid chromatography-mass spectrometry (LC-Orbitrap-MS) for component analysis using database software. Subsequently, a network approach was applied to pinpoint key anti-RA targets and pathways in order to validate its potential as an effective anti-RA agent. An adjuvant-induced arthritis (AIA) rat model was established, with oral administration of aqueous extract of EF over 30 consecutive days to evaluate its therapeutic effects. The results indicated that LC-Orbitrap-MS identified 29 principal compounds, including geniposidic acid, asperuloside, chlorogenic acid, kaempferol, aucubin, and trifolin. Pharmacological network analysis suggested that these components may exert their therapeutic effects on RA through modulation of NF-κB and PI3K-AKT signaling pathways. Furthermore, oral administration significantly reduced paw swelling in AIA rats while histological analysis demonstrated suppression of inflammatory responses in paw tissues by inhibiting NF-κB and PI3K-AKT pathways.