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Purpose <p>The present study investigates the purification of ACE-inhibitory sialoglycopeptides from sialoglycoprotein hydrolysate (SGPH) of rohu roe and evaluates their anticancer activity against the KB3-1 cancer cell line.</p> Methods <p>ACE-inhibitory sialoglycopeptides were purified sequentially from SGPH by QFF-ion exchange chromatography, Sephadex-G50 gel filtration and RP-HPLC. The anticancer activity of SGPH and the purified sialoglycopeptide fraction (RH-3P) was assessed in KB3-1 cells by DAPI and AO/EtBr staining, cell migration assay, and colony formation assay.</p> Result <p>QFF chromatogram depicted a single peak (QFF-P) with 63.46% ACE-inhibitory activity (5&#xa0;mg/ml). Gel filtration chromatography of QFF-P revealed 2 fractions, where GF-2 exhibited higher ACE-inhibitory activity of 49.71% (3&#xa0;mg/ml). RP-HPLC purification of GF-2 yielded 4 fractions, with RH-3 showing the highest ACE-inhibitory activity (47.61%) at 1&#xa0;mg/ml. Further, SGPH and purified sialoglycopeptide (RH-3P) inhibited the KB3-1 cancer cell line with IC₅₀ values of 7.1 and 6.8&#xa0;mg/ml, respectively (48&#xa0;h). DAPI and AO/EtBr staining of SGPH and RH-3P treated KB3-1 cells depicted morphological changes and early and late-stage apoptosis. Additionally, SGPH and RH-3P treated KB3-1 cells revealed inhibition of cell migration and reduction of KB3-1 colonies. These results illustrated that SGPH and RH-3P had anticancer effects against KB3-1 cells.</p> Conclusion <p>The results endorsed that SGPH and purified sialoglycopeptide can serve as ACE-inhibitory and anticancer agents in functional foods and nutraceutical products. Additionally, the use of rohu roes for bioactive SGPH production and sialoglycopeptide purification can help address fishery waste management challenges, minimize environmental pollution, and generate added income for fish farmers and the fisheries sector.</p> Graphical Abstract <p></p>

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Purification of ACE-Inhibitory Sialoglycopeptide from Sialoglycoprotein Hydrolysate of Labeo Rohita Roes and Evaluation of its Anticancer Potential

  • 期刊论文

  • 发表日期：

    2026年2月12日

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  • Anushma Venmarath,
  • Iyyannar Madhu,
  • Anbarasu Kannan,
  • Tanaji G. Kudre

摘要

Purpose

The present study investigates the purification of ACE-inhibitory sialoglycopeptides from sialoglycoprotein hydrolysate (SGPH) of rohu roe and evaluates their anticancer activity against the KB3-1 cancer cell line.

Methods

ACE-inhibitory sialoglycopeptides were purified sequentially from SGPH by QFF-ion exchange chromatography, Sephadex-G50 gel filtration and RP-HPLC. The anticancer activity of SGPH and the purified sialoglycopeptide fraction (RH-3P) was assessed in KB3-1 cells by DAPI and AO/EtBr staining, cell migration assay, and colony formation assay.

Result

QFF chromatogram depicted a single peak (QFF-P) with 63.46% ACE-inhibitory activity (5 mg/ml). Gel filtration chromatography of QFF-P revealed 2 fractions, where GF-2 exhibited higher ACE-inhibitory activity of 49.71% (3 mg/ml). RP-HPLC purification of GF-2 yielded 4 fractions, with RH-3 showing the highest ACE-inhibitory activity (47.61%) at 1 mg/ml. Further, SGPH and purified sialoglycopeptide (RH-3P) inhibited the KB3-1 cancer cell line with IC₅₀ values of 7.1 and 6.8 mg/ml, respectively (48 h). DAPI and AO/EtBr staining of SGPH and RH-3P treated KB3-1 cells depicted morphological changes and early and late-stage apoptosis. Additionally, SGPH and RH-3P treated KB3-1 cells revealed inhibition of cell migration and reduction of KB3-1 colonies. These results illustrated that SGPH and RH-3P had anticancer effects against KB3-1 cells.

Conclusion

The results endorsed that SGPH and purified sialoglycopeptide can serve as ACE-inhibitory and anticancer agents in functional foods and nutraceutical products. Additionally, the use of rohu roes for bioactive SGPH production and sialoglycopeptide purification can help address fishery waste management challenges, minimize environmental pollution, and generate added income for fish farmers and the fisheries sector.

Graphical Abstract