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Purpose <p>Scorpion venom contains various proteins and amino acids, which can be used to develop effective anti-venom medications. Bioinformatics technologies offer a valuable opportunity to study proteins based on their amino acid sequence. This work aimed to bioinformatically analyze the significant proteins in <i>Hottentotta saulcyi’s (H. saulcyi)</i> venom and assess its anticancer activity in the MDA-MB-231 cell line.</p> Method <p>In silico methodologies were used to understand the attributes of <i>H. saulcyi</i> venom peptides. The FASTA sequence of two peptides from the UniProt database was obtained. Bioinformatics tests including physicochemical characterization of venom peptides and secondary and tertiary structure prediction were performed. The MDA-MB-231 cell line was established, and MTT and Annexin V-PI tests were conducted to evaluate the toxicity of <i>H. saulcyi</i> scorpion venom. Quantitative Real-Time PCR analysis was conducted to assess the transcription of apoptosis-related genes P53, STAT3, and CDK7, together with the anti-apoptotic gene BCL2.</p> Results <p>Results showed that Toxin1 and cytochrome c oxidase subunit 1 exhibit stability, with Toxin1 being non-polar and cytochrome c oxidase subunit 1 being polar. MDA-MB-231 cells, when subjected to a dose of 5&#xa0;µg/mL, had survival rates of 27%, 41%, and 53% at 72&#xa0;h, 48&#xa0;h, and 24&#xa0;h, respectively. The results indicate that the survival of MDA-MB-231 cells significantly decreased at 72&#xa0;h (<i>P</i> &lt; 0.001). The real-time PCR results show that the groups that received <i>H. saulcyi</i> venom showed a notable rise in the transcription of proapoptotic genes (<i>P</i> &lt; 0.01). The examination of the transcription of the antiapoptotic gene <i>BCL2</i> was subsequently carried out among different groups. The expression of the <i>BCL2</i> gene was significantly higher in the PBS group compared to the venom treatment group (<i>P</i> &lt; 0.01).</p> Conclusion <p>These findings provide substantial insights into the molecular characteristics of <i>H. saulcyi</i> venom and its potential anticancer properties, therefore augmenting our understanding of venom-derived compounds in cancer research.</p>

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Modeling and Characterization of Hottentotta saulcyi Scorpion Venom and Investigation of Its Anticancer Properties

  • 期刊论文

  • 发表日期：

    2025年4月18日

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  • Mahshid Nosouhian,
  • Ali Asghar Rastegari,
  • Kahin Shahanipour,
  • Ali Mohammad Ahadi,
  • Mohammadreza Sheikh-Sajjadieh

摘要

Purpose

Scorpion venom contains various proteins and amino acids, which can be used to develop effective anti-venom medications. Bioinformatics technologies offer a valuable opportunity to study proteins based on their amino acid sequence. This work aimed to bioinformatically analyze the significant proteins in Hottentotta saulcyi’s (H. saulcyi) venom and assess its anticancer activity in the MDA-MB-231 cell line.

Method

In silico methodologies were used to understand the attributes of H. saulcyi venom peptides. The FASTA sequence of two peptides from the UniProt database was obtained. Bioinformatics tests including physicochemical characterization of venom peptides and secondary and tertiary structure prediction were performed. The MDA-MB-231 cell line was established, and MTT and Annexin V-PI tests were conducted to evaluate the toxicity of H. saulcyi scorpion venom. Quantitative Real-Time PCR analysis was conducted to assess the transcription of apoptosis-related genes P53, STAT3, and CDK7, together with the anti-apoptotic gene BCL2.

Results

Results showed that Toxin1 and cytochrome c oxidase subunit 1 exhibit stability, with Toxin1 being non-polar and cytochrome c oxidase subunit 1 being polar. MDA-MB-231 cells, when subjected to a dose of 5 µg/mL, had survival rates of 27%, 41%, and 53% at 72 h, 48 h, and 24 h, respectively. The results indicate that the survival of MDA-MB-231 cells significantly decreased at 72 h (P < 0.001). The real-time PCR results show that the groups that received H. saulcyi venom showed a notable rise in the transcription of proapoptotic genes (P < 0.01). The examination of the transcription of the antiapoptotic gene BCL2 was subsequently carried out among different groups. The expression of the BCL2 gene was significantly higher in the PBS group compared to the venom treatment group (P < 0.01).

Conclusion

These findings provide substantial insights into the molecular characteristics of H. saulcyi venom and its potential anticancer properties, therefore augmenting our understanding of venom-derived compounds in cancer research.