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Background <p>Several bioactive peptides derived from food sources exhibit high immunomodulatory activity. In our previous analyses, a potential rice-derived immunoregulatory peptide, GBP1 (NSVFRALPVDVVANAYR) was screened. However, its immunomodulatory function was not thoroughly investigated.</p> Purpose and Results <p>In this study, we explored the immunomodulatory activity of the GBP1 peptide using mouse bone marrow-derived dendritic cells (DC2.4). The results showed that GBP1 entered DC2.4 cells and stimulated their maturation. 10 μg/mL of GBP1 peptide promoted cell proliferation and maintained healthy cell morphology. Compared to the control group, the expression levels of CD86 and MHC II molecules in the 10 μg/mL GBP1 peptide group increased by 25.82% and 113%, respectively. Furthermore, treatment with GBP1 suppressed the mRNA expression of pro-inflammatory cytokines, including TNF-α and IFN-γ, while concurrently enhancing the expression of anti-inflammatory genes, such as IL-4 and IL-10. Molecular docking studies showed that the optimal conformation binding energy of GBP1 and MHC II was −11.5 kcal/mol, which could better present antigen information and exert immunomodulatory effects.</p> Conclusion <p>These findings suggest that the GBP1 peptide derived from rice processing by products could serve as a natural immunomodulatory alternative.</p>

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Evaluation of Immunoregulatory Functions of a Peptide From Rice: An in Silico and in Vitro Study

  • 期刊论文

  • 发表日期：

    2025年2月20日

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  • Ying Wu,
  • Zhimeng Zhang,
  • Zhimin Zhang,
  • Tingmin Qu,
  • Shiyu Wen,
  • Hao Wu,
  • Mao-Long Chen,
  • Li Ding,
  • Yufei Xie,
  • Qingming Huang,
  • Jian Hu,
  • Li Wen

摘要

Background

Several bioactive peptides derived from food sources exhibit high immunomodulatory activity. In our previous analyses, a potential rice-derived immunoregulatory peptide, GBP1 (NSVFRALPVDVVANAYR) was screened. However, its immunomodulatory function was not thoroughly investigated.

Purpose and Results

In this study, we explored the immunomodulatory activity of the GBP1 peptide using mouse bone marrow-derived dendritic cells (DC2.4). The results showed that GBP1 entered DC2.4 cells and stimulated their maturation. 10 μg/mL of GBP1 peptide promoted cell proliferation and maintained healthy cell morphology. Compared to the control group, the expression levels of CD86 and MHC II molecules in the 10 μg/mL GBP1 peptide group increased by 25.82% and 113%, respectively. Furthermore, treatment with GBP1 suppressed the mRNA expression of pro-inflammatory cytokines, including TNF-α and IFN-γ, while concurrently enhancing the expression of anti-inflammatory genes, such as IL-4 and IL-10. Molecular docking studies showed that the optimal conformation binding energy of GBP1 and MHC II was −11.5 kcal/mol, which could better present antigen information and exert immunomodulatory effects.

Conclusion

These findings suggest that the GBP1 peptide derived from rice processing by products could serve as a natural immunomodulatory alternative.