Study of Image Processing Method for Chronic Prostatitis-Chronic Pelvic Pain Syndrome (CP/CPPS) Mice Model

We determine the prostatic interstitium area, the inflammation’s localization, and the impact of different doses on the group of model mice.

A total of 30 male ICR mice were randomly grouped into 5 (n = 6): 45 µg group = 6, 60 µg group = 6, 90 µg group = 6, 120 µg group = 6, 120 µg group = 6, control group = 6. With the exception of the control group, all the groups were immunized by injecting 0.2 ml of T2 peptide emulsion and immune adjuvant CFA to induce non-bacterial chronic prostatitis on days 0 and 14 of the mice and finally executed on day 28. All injections were administered subcutaneously. HE staining was used to evaluate changes in prostate pathology. Image J was used to calculate the area of the prostate interstitium, which represents the degree of prostate edema. To compare statistical differences between groups, the ANOVA test was used.

From the perspective of pathological scoring, the 60 µg, 90 µg, and 120 µg groups had the highest scores by using Image J to treat inflammatory cells. Also, in the prostate interstitium area treated, it was found that the 90ug group attained the largest prostate interstitial area as well as the highest degree of swelling.

From the results, Image J software is an effective tool in the calculating the surface of the prostatic interstitium and the specific localization of the inflammation.