Context <p>Natural and anthropogenic disturbance can influence the connectivity of riparian forest ecosystems affecting the movement of terrestrial vertebrates.</p> Objectives <p>We examined changes in connectivity of riparian forest habitat for terrestrial vertebrates following natural and anthropogenic disturbance between 2000 and 2021.</p> Methods <p>We quantified changes in patch aggregation and used a circuit-theory model (Omniscape) to quantify changes in connectivity of riparian forest habitat within three hypothetical search distances representing the movement capabilities of terrestrial vertebrates (0–500, 0–1000, 0–2000&#xa0;m). We replicated the analysis for six (&gt; 10,000&#xa0;km²) landscapes (i.e., provincial ecosections) in British Columbia, Canada. We used random forest regression to quantify hypothesized relationships between change in habitat connectivity and explanatory variables known to influence disturbance: topography, types of aquatic features (streams, lakes and wetlands), and management regime (industrial forest or protected area).</p> Results <p>Loss of forest across landscapes ranged from 3.1% to 12.3%. Changes in forest aggregation were consistent with clustered patterns of disturbances such as wildfire and large-scale outbreaks of bark beetles. Topography (elevation, slope, aspect) influenced changes in connectivity of riparian forest habitat. Loss of connectivity was greatest around small streams (&lt; 5&#xa0;m wide) within the finest search distance (500&#xa0;m).</p> Conclusions <p>The maintenance of forest immediately adjacent to water features, and in particular small streams, is important for ensuring the connectivity of riparian forest habitat for terrestrial vertebrates.</p>