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<p>Polysaccharides derived from <i>Pleurotus eryngii</i> (<i>P. eryngii</i>), among the most important bioactive components found in mushrooms, exhibit a range of health-promoting functions. This study focuses on cultivating probiotic strains, namely <i>Lactobacillus plantarum</i> (<i>L. plantarum</i>) and <i>Kluyveromyces marxianus</i> (<i>K. marxianus</i>). It investigates their growth and proliferation in the presence of <i>P. eryngii</i> polysaccharides (PEP). In addition, the influence of different irradiation sources (<i>γ</i>-rays, pulsed light, light-emitting diodes, LEDs, and cold cathode fluorescent lamps, CCFLs) and doses on irradiated PEP and their subsequent effects on microbial growth were examined. Irradiation altered the molecular structure of PEP, as reflected in changes in moisture desorption, gelation, and thermal decomposition behavior determined by differential scanning calorimetry (DSC). Non-irradiated and light-irradiated PEP functioned as utilizable carbon sources in the medium for <i>L. plantarum</i> under the present in vitro conditions, as demonstrated by isothermal microcalorimetry (TAM Air) tests. The growth curves indicated that <i>L. plantarum</i> can metabolize <i>P. eryngii</i> polysaccharides, although the magnitude of growth enhancement depended on the glucose concentration and irradiation dose. In control groups, media containing 1% glucose generally outperformed PEP-only media, suggesting that excessively high sugar concentrations may hinder growth. In contrast, complete replacement of glucose by PEP (PEP2) led to lower growth enthalpy than glucose-containing controls. Furthermore, adding PEP did not significantly enhance growth in <i>K. marxianus</i> medium, particularly when glucose was replaced entirely. Overall, PEP appears suitable for partial replacement of glucose as a carbon source in the medium for <i>L. plantarum</i> under these experimental conditions. In contrast, complete replacement resulted in reduced growth compared with glucose-containing controls. <i>K. marxianus</i> was more sensitive to carbon source selection, and the glucose concentration in the medium directly affected its growth. These findings are based on single strains of <i>L. plantarum</i> and <i>K. marxianus</i> in controlled in vitro cultures, and further in vivo and multi-strain studies are required before concluding prebiotic effects in humans.</p>

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Assessing the prebiotic effects on microorganism growth through various irradiation treatments of Pleurotus eryngii polysaccharides using microcalorimetry

  • 期刊论文

  • 发表日期：

    2026年1月4日

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  • Shu-Yao Tsai,
  • Hei-Yiu So,
  • Chun-Ping Lin

摘要

Polysaccharides derived from Pleurotus eryngii (P. eryngii), among the most important bioactive components found in mushrooms, exhibit a range of health-promoting functions. This study focuses on cultivating probiotic strains, namely Lactobacillus plantarum (L. plantarum) and Kluyveromyces marxianus (K. marxianus). It investigates their growth and proliferation in the presence of P. eryngii polysaccharides (PEP). In addition, the influence of different irradiation sources (γ-rays, pulsed light, light-emitting diodes, LEDs, and cold cathode fluorescent lamps, CCFLs) and doses on irradiated PEP and their subsequent effects on microbial growth were examined. Irradiation altered the molecular structure of PEP, as reflected in changes in moisture desorption, gelation, and thermal decomposition behavior determined by differential scanning calorimetry (DSC). Non-irradiated and light-irradiated PEP functioned as utilizable carbon sources in the medium for L. plantarum under the present in vitro conditions, as demonstrated by isothermal microcalorimetry (TAM Air) tests. The growth curves indicated that L. plantarum can metabolize P. eryngii polysaccharides, although the magnitude of growth enhancement depended on the glucose concentration and irradiation dose. In control groups, media containing 1% glucose generally outperformed PEP-only media, suggesting that excessively high sugar concentrations may hinder growth. In contrast, complete replacement of glucose by PEP (PEP2) led to lower growth enthalpy than glucose-containing controls. Furthermore, adding PEP did not significantly enhance growth in K. marxianus medium, particularly when glucose was replaced entirely. Overall, PEP appears suitable for partial replacement of glucose as a carbon source in the medium for L. plantarum under these experimental conditions. In contrast, complete replacement resulted in reduced growth compared with glucose-containing controls. K. marxianus was more sensitive to carbon source selection, and the glucose concentration in the medium directly affected its growth. These findings are based on single strains of L. plantarum and K. marxianus in controlled in vitro cultures, and further in vivo and multi-strain studies are required before concluding prebiotic effects in humans.