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<p>The extensive use of uranium resources has increased radionuclide wastes, necessitating efficient, economical, and environmentally remediation strategies. Here, a biosurfactant-producing <i>Pseudomonas aeruginosa</i> strain was isolated from soil. Optimal fermentation conditions were 35&#xa0;°C, pH7.0, 2% glycerol, and 0.1% ammonium nitrate, yielding 0.24&#xa0;g&#xa0;L<sup>−1</sup> biosurfactant. The product remained stable at extreme environments; Fourier Transform Infrared Spectroscopy&#xa0;(FTIR) identified it as rhamnolipid. In 10&#xa0;mg&#xa0;L<sup>−1</sup> uranium removal assays, biosurfactant plus cells achieved 90.16% uranium removal at 120&#xa0;min, outperforming single treatments.</p>

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Bioremediation of uranium contamination by biosurfactant-producing Pseudomonas aeruginosa

  • 期刊论文

  • 发表日期：

    2026年3月27日

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  • Yuping Deng,
  • Yingying Jia,
  • Shunxi Yang,
  • Fangzhu Xiao

摘要

The extensive use of uranium resources has increased radionuclide wastes, necessitating efficient, economical, and environmentally remediation strategies. Here, a biosurfactant-producing Pseudomonas aeruginosa strain was isolated from soil. Optimal fermentation conditions were 35 °C, pH7.0, 2% glycerol, and 0.1% ammonium nitrate, yielding 0.24 g L−1 biosurfactant. The product remained stable at extreme environments; Fourier Transform Infrared Spectroscopy (FTIR) identified it as rhamnolipid. In 10 mg L−1 uranium removal assays, biosurfactant plus cells achieved 90.16% uranium removal at 120 min, outperforming single treatments.