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<p>We have synthesized <sup>68</sup>Ga labeled citrate capped silver (Ag) nanoparticles (NPs) based formulation for the diagnosis of <i>Staphylococcus aureus</i> (<i>S</i>. <i>aureus</i>) bacterial infection induced in Wistar rats by employing PET/CT imaging. Initially, citrtate capped Ag NPs were synthesized and characterized by powder XRD, SEM, EDS and TEM analyses as well as by FT-IR and UV–Vis spectroscopy. The TEM analyses revealed their average particle size of ~ 6&#xa0;nm. The SAED pattern indicated crystallinity of Ag NPs. The DLS mesurements revealed the hydrodynamic size of ~ 30–35&#xa0;nm and zeta potential of − 26.72&#xa0;mV ± 1.56 was found. Later, the <sup>68</sup>Ga labeling was performed through <i>in-situ</i> chelation of <sup>68</sup>GaCl<sub>3</sub> with carboxylate groups of trisodium citrate, which were capped on Ag NPs. The radiolabeling yield was found to be ~ 80% and the stability evaluation revealed ~ 90% stability in saline and ~ 78% stability in plasma. The <i>in-vivo</i> PET/CT imaging by <sup>68</sup>Ga labeled Ag NPs formulation could detect inflammation induced by <i>S. aureus</i> infection in Wistar rats which was compared with PET/CT image after administering <sup>18</sup>F-FDG, a clinical probe and it reveled that the presented Ag NPs based formulation is appropriate for PET/CT imaging. Aditionally, the synthesis of the presented test formulation was facile also.</p>

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Development of 68Ga labeled silver nanoparticles based multimodal formulation for in-vivo diagnosis of Staphylococcus aureus infection in Wistar rats

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Prasad P. Phadnis,
  • Shishu Kant Suman,
  • Chandan Kumar,
  • Lalrinawma Zote,
  • Asheesh Kumar,
  • Jitendra Bahadur,
  • Sutapa Rakshit,
  • Avik Chakraborty

摘要

We have synthesized 68Ga labeled citrate capped silver (Ag) nanoparticles (NPs) based formulation for the diagnosis of Staphylococcus aureus (S. aureus) bacterial infection induced in Wistar rats by employing PET/CT imaging. Initially, citrtate capped Ag NPs were synthesized and characterized by powder XRD, SEM, EDS and TEM analyses as well as by FT-IR and UV–Vis spectroscopy. The TEM analyses revealed their average particle size of ~ 6 nm. The SAED pattern indicated crystallinity of Ag NPs. The DLS mesurements revealed the hydrodynamic size of ~ 30–35 nm and zeta potential of − 26.72 mV ± 1.56 was found. Later, the 68Ga labeling was performed through in-situ chelation of 68GaCl3 with carboxylate groups of trisodium citrate, which were capped on Ag NPs. The radiolabeling yield was found to be ~ 80% and the stability evaluation revealed ~ 90% stability in saline and ~ 78% stability in plasma. The in-vivo PET/CT imaging by 68Ga labeled Ag NPs formulation could detect inflammation induced by S. aureus infection in Wistar rats which was compared with PET/CT image after administering 18F-FDG, a clinical probe and it reveled that the presented Ag NPs based formulation is appropriate for PET/CT imaging. Aditionally, the synthesis of the presented test formulation was facile also.