<p>In situ treatment of uranium-containing wastewater from uranium mining areas remains a significant challenge. Herein, microorganisms resistance to uranium (VI) were isolated. Subsequently, sand column experiments were carried out using local ore as a carrier to immobilize the isolated microorganisms. The results demonstrated that the ore can effectively support a high microbial loading and exhibited favorable biocompatibility. Compared with the ore alone, the immobilized microorganisms displayed a more rapid and stable capacity for uranium transformation and removal. While the maximum removal efficiency of the ore alone reached only 50%, while that of the immobilized microorganisms achieved up to 99%. Furthermore, the influence of various reaction parameters on uranium removal performance was systematically investigated. Under optimal conditions of pH 7, temperature of 25°C, initial uranium concentration of 20 mg/L, and inoculation ratio of 10%, the removal efficiency of the ore-immobilized microorganisms reached 99.4%, with a residual uranium concentration of 0.12 mg/L. The mechanisms revealed that in-situ cultivated microorganisms efficiently converted soluble uranium (VI) into insoluble forms and stable adsorbed uranium (VI) ions, thereby significantly enhancing the uranium immobilization capacity of the ore carrier. The immobilized microorganisms reduced soluble uranium (VI) to insoluble U(IV) precipitates via biological reduction and other resistance pathways, achieving a level of uranium removal that far exceeded the adsorption capacity of the ore alone. These findings provide a solid theoretical foundation and reliable technical support for the efficient, stable, and environmentally sustainable bioremediation of uranium-containing wastewater.</p>

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In situ microbial remediation of uranium-contaminated wastewater in stabilized uranium mining areas

  • Jianming Li,
  • Haotong Guo,
  • Zhiwu Lei,
  • Eming Hu,
  • Boyuan Zheng,
  • Jinming Hu,
  • Qingliang Wang

摘要

In situ treatment of uranium-containing wastewater from uranium mining areas remains a significant challenge. Herein, microorganisms resistance to uranium (VI) were isolated. Subsequently, sand column experiments were carried out using local ore as a carrier to immobilize the isolated microorganisms. The results demonstrated that the ore can effectively support a high microbial loading and exhibited favorable biocompatibility. Compared with the ore alone, the immobilized microorganisms displayed a more rapid and stable capacity for uranium transformation and removal. While the maximum removal efficiency of the ore alone reached only 50%, while that of the immobilized microorganisms achieved up to 99%. Furthermore, the influence of various reaction parameters on uranium removal performance was systematically investigated. Under optimal conditions of pH 7, temperature of 25°C, initial uranium concentration of 20 mg/L, and inoculation ratio of 10%, the removal efficiency of the ore-immobilized microorganisms reached 99.4%, with a residual uranium concentration of 0.12 mg/L. The mechanisms revealed that in-situ cultivated microorganisms efficiently converted soluble uranium (VI) into insoluble forms and stable adsorbed uranium (VI) ions, thereby significantly enhancing the uranium immobilization capacity of the ore carrier. The immobilized microorganisms reduced soluble uranium (VI) to insoluble U(IV) precipitates via biological reduction and other resistance pathways, achieving a level of uranium removal that far exceeded the adsorption capacity of the ore alone. These findings provide a solid theoretical foundation and reliable technical support for the efficient, stable, and environmentally sustainable bioremediation of uranium-containing wastewater.