Research on urease-modified Deinococcus radiodurans and the control mechanism of the contamination of U(VI)

Biosorption by microorganisms is an environmentally friendly and efficient method to adsorb heavy metals and radionuclieds. The purpose of this study was to transform urease gene (Ure) from Sporosarcina pasteurii DSM33 into D.radiodurans to prepare a recombinant Deino-Ure strains. The urease was used to decompose urea to produce CO₃²⁻, promoting calcium carbonate precipitation in the presence of Ca²⁺. This enhances the biomineralisation and U(IV) enrichment of D. radiodurans, which provides a scientific basis for the treatment of low enriched uranium contaminated soil. During Deino-Ure mineralization of U(VI), uranium may co-precipitate with calcium, forming a stable U(VI)/U (IV)-calcite mineral precipitate.