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Response of soil bacteria to PUREX chemicals suggests biomarker utility and bioremediation potential

  • Justin C. Podowski,
  • Sara Forrester,
  • Dionysios A. Antonopoulos,
  • Jennifer L. Steeb,
  • Angela D. Kent,
  • James J. Davis,
  • Daniel S. Schabacker

摘要

Chemicals involved in plutonium uranium reduction extraction (PUREX) can be released from nuclear reprocessing facilities and accumulate in the environment. We exposed chemically diverse soils to a range of concentrations of key chemicals used in the PUREX process. The responses of soil microbial communities are dependent on soil type, and tributyl phosphate exposure generates the most reproducible changes in microbial communities. We reconstructed the genomes of key bacteria and find several phosphotriesterase genes found only in Rhizobiaceae. The abundance of phosphotriesterase genes is significantly higher in samples exposed to tributyl phosphate. These phosphotriesterase genes may be involved in breakdown of tributyl phosphate, and a means of accessing phosphate for these bacteria.