New approach for in-house production of [⁶⁸Ga]Ga-NOTA-UBI_(29–41) using a titanium dioxide column-based generator

Efforts have been dedicated to distinguishing between infections and sterile inflammations. This study presents a novel approach for in-house synthesis of [⁶⁸Ga]Ga-NOTA-UBI_(29–41) employing a titanium dioxide column-based ⁶⁸Ga-generator. The optimized method yielded the radiopeptide with high radiochemical purity (> 99%) and stability (up to 90 min). [⁶⁸Ga]Ga-NOTA-UBI_(29–41) demonstrates hydrophilic characteristics (Log P = − 3.57 ± 0.20) and a binding to serum proteins of ~ 60%. The affinity of [⁶⁸Ga]Ga-NOTA-UBI_(29–41) to bacteria was directly proportional to the increasing quantity of bacterial cells. Consequently, this method generates [⁶⁸Ga]Ga-NOTA-UBI_(29–41) with remarkable physicochemical characteristics and high binding affinity to bacterial cells, rendering it suitable for clinical applications.