<p>Molecular imprinting typically relies on highly crosslinked polymer networks to preserve template-derived binding sites. In this study, we provide a proof-of-concept for template-dependent rebinding signals in non-network protein-templated phenolic copolymers and evaluate these signals using a chemiluminescent immunoassay. Phenolic copolymers were synthesized by horseradish peroxidase-catalyzed oxidative copolymerization of 4-tert-butylphenol and tyramine in the presence or absence of a protein template, followed by different purification procedures. Under the main condition (HCl treatment and a 4-tert-butylphenol/tyramine ratio of 9:1), the templated polymer showed a significantly higher signal than the non-templated control in the anti-BSA assay after incubation with bovine serum albumin. An exploratory survey across purification methods and monomer ratios further indicated that the observed signal difference was strongly dependent on processing conditions. Although the present study does not provide direct proof of specific template-derived binding and residual template protein cannot be fully excluded as a contributing factor, the results suggest that non-network phenolic copolymer systems can exhibit condition-dependent signal differences consistent with template-dependent rebinding. These findings provide a starting point for exploring non-network phenolic copolymers as an alternative design space to conventional network-based molecularly imprinted materials.</p>