<p>Poly(vinyl chloride) (PVC) is a type of thermoplastic polymer of major importance. It is extremely rigid, making it unsuitable for some applications; therefore, PVC is often plasticized. One of the key drawbacks of utilizing synthetic plasticizers in PVC as a material for blood bags is the toxicity of the plasticizers. To address this, the present study aimed to utilize natural materials as plasticizers for PVC. Two types of essential oil (rosemary and pine oil) were added to PVC in two concentrations (1% and 2%) using the solvent casting method. The mechanical and physical properties, functional groups, wettability, and hemolytic, antibacterial, transparency and thermal stability characteristics of the obtained materials were evaluated. Favorable effects of rosemary essential oil addition included reductions in the ultimate tensile strength and modulus of elasticity and an increase in elongation. The favorable reductions in tensile strength and elasticity obtained at 2% rosemary oil addition were 54.19% and 59.8%, respectively, while the elongation percentage increased to 97.58%. In addition, the transparency and water absorption decreased with increasing additive concentrations. Furthermore, the largest Staphylococcus aureus growth inhibition zones were recorded with a rise in essential oil content, with increases of 44.44% for 2% rosemary oil and 66.67% for 2% pine oil. Also, the added oil led to the raise the thermal stabilizer. The obtained results were compared with traditional blood bags available at Iraqi hospitals, with the prepared composites found to be the best alternative to conventional materials. Finally, all the data were statistically analyzed and found to be significant at a level of <i>p</i> &lt; 0.05.</p>