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<p>A temperature and pH-sensitive biodegradable cationic amphiphilic copolymer, poly [2-hydroxyethyl methacrylate-caprolactone-dimethylamine ethyl methacrylate quaternary ammonium alkyl halide] [P(HEMA-PCL-MADQUAT)], was synthesized via ring-opening polymerization (ROP) and free radical polymerization methods. Structure confirmation was obtained by FT-IR and <sup>1</sup>H-NMR spectroscopy. Nano-micelles derived from the copolymer were characterized by TEM (transmission electron microscopy), DLS (dynamic light scattering), zeta potential, and CMC (critical micelle concentration) analysis, revealing a uniform morphology with a surface charge of − 12.4 mV, an average diameter of 280&#xa0;nm, and a CMC of 0.01&#xa0;g/L. The micelles efficiently encapsulated methotrexate and ciprofloxacin, with loading efficiencies of 67.1% and 71.4%, respectively. MTT assays in MCF-7 cell lines demonstrated favorable biocompatibility and CIP@MTX micelles showed lower IC₅₀ than free CIP@MTX in MCF-7 cells (1.5 vs. 5.3&#xa0;µg·mL⁻¹). These findings highlight P(HEMA-PCL-MADQUAT) micelles as a promising platform for breast cancer therapy, combining effective drug delivery with reduced side effects.</p>

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Stimuli responsive polymeric micelles for combinational delivery and quantification of methotrexate and ciprofloxacin based on high performance liquid chromatography

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Mahdi Abadi Gharehbaba,
  • Majid Soleimani,
  • Samira Jafari

摘要

A temperature and pH-sensitive biodegradable cationic amphiphilic copolymer, poly [2-hydroxyethyl methacrylate-caprolactone-dimethylamine ethyl methacrylate quaternary ammonium alkyl halide] [P(HEMA-PCL-MADQUAT)], was synthesized via ring-opening polymerization (ROP) and free radical polymerization methods. Structure confirmation was obtained by FT-IR and 1H-NMR spectroscopy. Nano-micelles derived from the copolymer were characterized by TEM (transmission electron microscopy), DLS (dynamic light scattering), zeta potential, and CMC (critical micelle concentration) analysis, revealing a uniform morphology with a surface charge of − 12.4 mV, an average diameter of 280 nm, and a CMC of 0.01 g/L. The micelles efficiently encapsulated methotrexate and ciprofloxacin, with loading efficiencies of 67.1% and 71.4%, respectively. MTT assays in MCF-7 cell lines demonstrated favorable biocompatibility and CIP@MTX micelles showed lower IC₅₀ than free CIP@MTX in MCF-7 cells (1.5 vs. 5.3 µg·mL⁻¹). These findings highlight P(HEMA-PCL-MADQUAT) micelles as a promising platform for breast cancer therapy, combining effective drug delivery with reduced side effects.