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<p>Nanotechnology-based drug delivery systems offer promising advancements in targeted cancer therapy. This study focuses on the preparation and evaluation of quercetin-loaded nanocarriers composed of chitosan, polyvinyl alcohol (PVA), and aluminum oxide (γ-alumina) as a pH-sensitive delivery system that enhances the therapeutic efficacy of quercetin against cancer cells, particularly MCF-7 breast cancer cells. To characterize the structural and physicochemical properties of the nanocomposites, field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared (FT-IR) spectroscopy, and dynamic light scattering (DLS) were employed. The results of the analyses indicate the presence of aliphatic C–H and O–H bonds in spherical nanocomposite particles with a smooth surface. Drug release studies demonstrated a sustained release profile over 96&#xa0;h, with a higher release rate at pH 5.4 compared to pH 7.4. MTT assay results showed that the CS-PVA-Al<sub>2</sub>O<sub>3</sub>@QC formulation exhibited the highest cytotoxicity against MCF-7 cancer cells, significantly greater than both CS-PVA-Al<sub>2</sub>O<sub>3</sub> and free quercetin (P &lt; 0.05). In contrast, most formulations showed no significant toxicity toward L929 healthy cells (P &gt; 0.05). Flow cytometry analysis revealed significant apoptosis induction in MCF-7 cells treated with the CS-γ-Al<sub>2</sub>O<sub>3</sub>-PVA-QC nanocarrier, with early apoptosis observed in 64.5% of cells compared to 7.96% for free quercetin. These results suggest that this nanocarrier the chitosan/PVA/γ-alumina nanocomposite presents a novel and effective pH-sensitive drug delivery system with significant potential for anticancer applications.</p>

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Cytotoxic Assessment and Characterization of Chitosan/Polyvinyl Alcohol/γ-Alumina Nanocomposite for Quercetin Delivery, a Potential for Cancer Treatment

  • 期刊论文

  • 发表日期：

    2026年6月14日

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  • Hajar Afshar,
  • Mehrab Pourmadadi,
  • Fatemeh Yazdian,
  • Ahmad Hallajisani

摘要

Nanotechnology-based drug delivery systems offer promising advancements in targeted cancer therapy. This study focuses on the preparation and evaluation of quercetin-loaded nanocarriers composed of chitosan, polyvinyl alcohol (PVA), and aluminum oxide (γ-alumina) as a pH-sensitive delivery system that enhances the therapeutic efficacy of quercetin against cancer cells, particularly MCF-7 breast cancer cells. To characterize the structural and physicochemical properties of the nanocomposites, field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared (FT-IR) spectroscopy, and dynamic light scattering (DLS) were employed. The results of the analyses indicate the presence of aliphatic C–H and O–H bonds in spherical nanocomposite particles with a smooth surface. Drug release studies demonstrated a sustained release profile over 96 h, with a higher release rate at pH 5.4 compared to pH 7.4. MTT assay results showed that the CS-PVA-Al2O3@QC formulation exhibited the highest cytotoxicity against MCF-7 cancer cells, significantly greater than both CS-PVA-Al2O3 and free quercetin (P < 0.05). In contrast, most formulations showed no significant toxicity toward L929 healthy cells (P > 0.05). Flow cytometry analysis revealed significant apoptosis induction in MCF-7 cells treated with the CS-γ-Al2O3-PVA-QC nanocarrier, with early apoptosis observed in 64.5% of cells compared to 7.96% for free quercetin. These results suggest that this nanocarrier the chitosan/PVA/γ-alumina nanocomposite presents a novel and effective pH-sensitive drug delivery system with significant potential for anticancer applications.