Scholarlink

<p>Exopolysaccharides (EPS) produced by&#xa0;<i>Paenibacillus mucilaginosus</i>&#xa0;are promising biopolymers with potential applications in agricultural, biotechnological, and industrial fields. This study investigated how supplementing the growth medium with Moroccan clay influences EPS production and properties compared with a clay-free control culture. EPS obtained from both conditions were characterized using scanning electron microscopy (SEM) to examine surface morphology, energy-dispersive X-ray (EDX) spectroscopy to determine elemental composition, and high-performance liquid chromatography (HPLC) to quantify monosaccharide profiles. Additional structural information was acquired by Fourier transform infrared (FTIR) spectroscopy, two-dimensional proton NMR (NOESY), and transmission electron microscopy (TEM) of bacterial cells and their surrounding polymer matrix. Relative to the control, the clay-supplemented cultures showed higher EPS-associated sugar content and noticeable differences in sugar profiles, elemental signals, and microstructural organization of the polymer. These observations indicate that Moroccan clay acts as a mineral additive that modifies the physicochemical environment and is associated with a more compact and mineral-associated EPS matrix. The findings highlight the potential of Moroccan clay to modulate microbial EPS characteristics in ways that may be useful for future agricultural and industrial applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Characterization of Exopolysaccharide Structural Modifications Induced by Morocco Clay in Glycerol Medium produced by Paenibacillus mucilaginosus

  • 期刊论文

  • 发表日期：

    2026年6月30日

查看全文
  • Shimaa K. Ali,
  • Eman A. Hamed,
  • Basma H. Amin,
  • Mona A. A. Kishk,
  • Afrah E. Mohammed,
  • Seham M. Hamed,
  • Hussein S. Mohamed

摘要

Exopolysaccharides (EPS) produced by Paenibacillus mucilaginosus are promising biopolymers with potential applications in agricultural, biotechnological, and industrial fields. This study investigated how supplementing the growth medium with Moroccan clay influences EPS production and properties compared with a clay-free control culture. EPS obtained from both conditions were characterized using scanning electron microscopy (SEM) to examine surface morphology, energy-dispersive X-ray (EDX) spectroscopy to determine elemental composition, and high-performance liquid chromatography (HPLC) to quantify monosaccharide profiles. Additional structural information was acquired by Fourier transform infrared (FTIR) spectroscopy, two-dimensional proton NMR (NOESY), and transmission electron microscopy (TEM) of bacterial cells and their surrounding polymer matrix. Relative to the control, the clay-supplemented cultures showed higher EPS-associated sugar content and noticeable differences in sugar profiles, elemental signals, and microstructural organization of the polymer. These observations indicate that Moroccan clay acts as a mineral additive that modifies the physicochemical environment and is associated with a more compact and mineral-associated EPS matrix. The findings highlight the potential of Moroccan clay to modulate microbial EPS characteristics in ways that may be useful for future agricultural and industrial applications.