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<p>Endophytic fungi and their secondary metabolites represent a valuable microbial resource with diverse bioactivities and potential applications. In this study, <i>Talaromyces</i> sp. CCTCC M2025051 was isolated from wheat bran, and its extracellular polysaccharide (TSP) was investigated. Compositional analysis revealed that the yield of TSP was 3.34 ± 0.17&#xa0;g/L, comprising 93.11 ± 2.93% carbohydrate and 2.82 ± 0.07% protein. TSP was identified as a heteropolysaccharide composed of arabinose, galactose, glucose, mannose, and glucuronic acid in a molar ratio of 0.0985: 4.0208: 35.6949: 2.0528: 0.1718, with a molecular weight of 235.187&#xa0;kDa and a polydispersity of 1.886. In vitro assays demonstrated that TSP effectively scavenged ABTS, DPPH, hydroxyl, and superoxide radicals in a concentration-dependent manner. Furthermore, TSP exhibited significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). This effect was accompanied by the modulation of oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). This work provides a scientific basis for the potential application of TSP as an antioxidant and anti-inflammatory agent.</p>

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Structural Characterization and Anti-inflammatory Activity of a Polysaccharide Produced by Endophytic Fungus Talaromyces Sp. CCTCC M 2025051

  • 期刊论文

  • 发表日期：

    2025年9月8日

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  • Zichao Wang,
  • Yixin Shi,
  • Xin Liu,
  • Na Li,
  • Jiaqi Wang,
  • Hongtao Zhang,
  • Yingjie Feng,
  • Yongming Xu,
  • Qinggong Chen,
  • Changxing Liu,
  • Tao Liu,
  • Dandan Liu,
  • Qi Wang,
  • Jinchu Yang,
  • Lemei An

摘要

Endophytic fungi and their secondary metabolites represent a valuable microbial resource with diverse bioactivities and potential applications. In this study, Talaromyces sp. CCTCC M2025051 was isolated from wheat bran, and its extracellular polysaccharide (TSP) was investigated. Compositional analysis revealed that the yield of TSP was 3.34 ± 0.17 g/L, comprising 93.11 ± 2.93% carbohydrate and 2.82 ± 0.07% protein. TSP was identified as a heteropolysaccharide composed of arabinose, galactose, glucose, mannose, and glucuronic acid in a molar ratio of 0.0985: 4.0208: 35.6949: 2.0528: 0.1718, with a molecular weight of 235.187 kDa and a polydispersity of 1.886. In vitro assays demonstrated that TSP effectively scavenged ABTS, DPPH, hydroxyl, and superoxide radicals in a concentration-dependent manner. Furthermore, TSP exhibited significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). This effect was accompanied by the modulation of oxidative stress markers, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). This work provides a scientific basis for the potential application of TSP as an antioxidant and anti-inflammatory agent.