Scholarlink

<p>World population growth associated with unbridled consumption have led to notable increases in the use of petroleum-based plastics. Biodegradable polymers produced from sustainable and low-cost raw materials have been emerging as a key solution. The objective of this work was to obtain a polymer of microbial origin from an alternative and renewable source. Bacterial Polyhydroxybutyrate (PHB-B) was produced by submerged culture of <i>Cupriavidus necator</i> using commercial sugarcane molasses (CSM) as a renewable substrate. A 2<sup>2</sup> factorial experimental design with three central point replications was conducted to identify the most favorable culture conditions. The 15&#xa0;g.L<sup>−1</sup> concentration of reducing sugar (RS) obtained from the CSM was effective as a carbon source, yielding (under stirring at 180&#xa0;rpm and 30&#xa0;°C) a maximum production of 35.70% PHB-B, while presenting the same functional groups and crystal structure as commercial grade Polyhydroxybutyrate (PHB-C). The intensities obtained for the 2933 and 2975&#xa0;cm<sup>−1</sup> bands, suggested that PHB-C presents higher crystallinity than PHB-B. <sup>1</sup>H and <sup>13</sup>C NMR confirmed the identity of the bacterial biopolymer as Polyhydroxybutyrate. The diffraction peaks were less intense for PHB-B, and the peaks at (021) and (101) coalesced into a single peak at (111). The peak shapes indicated that PHB-B presents smaller and/or less perfect crystals than PHB-C. Considering the observed structural differences, the rigidity and flexibility of the biopolymers may differ. Bacterial PHB was produced using a low-pollution route. This resulted in a product which potentially expands the range of environmentally friendly PHB applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological Production and Structural Characterization of PHB from Commercial Sugarcane Molasses

  • 期刊论文

  • 发表日期：

    2025年1月31日

查看全文
  • Débora J. N. de Melo,
  • Lucineide B. da Silva,
  • Sharline F. M. Santos

摘要

World population growth associated with unbridled consumption have led to notable increases in the use of petroleum-based plastics. Biodegradable polymers produced from sustainable and low-cost raw materials have been emerging as a key solution. The objective of this work was to obtain a polymer of microbial origin from an alternative and renewable source. Bacterial Polyhydroxybutyrate (PHB-B) was produced by submerged culture of Cupriavidus necator using commercial sugarcane molasses (CSM) as a renewable substrate. A 22 factorial experimental design with three central point replications was conducted to identify the most favorable culture conditions. The 15 g.L−1 concentration of reducing sugar (RS) obtained from the CSM was effective as a carbon source, yielding (under stirring at 180 rpm and 30 °C) a maximum production of 35.70% PHB-B, while presenting the same functional groups and crystal structure as commercial grade Polyhydroxybutyrate (PHB-C). The intensities obtained for the 2933 and 2975 cm−1 bands, suggested that PHB-C presents higher crystallinity than PHB-B. 1H and 13C NMR confirmed the identity of the bacterial biopolymer as Polyhydroxybutyrate. The diffraction peaks were less intense for PHB-B, and the peaks at (021) and (101) coalesced into a single peak at (111). The peak shapes indicated that PHB-B presents smaller and/or less perfect crystals than PHB-C. Considering the observed structural differences, the rigidity and flexibility of the biopolymers may differ. Bacterial PHB was produced using a low-pollution route. This resulted in a product which potentially expands the range of environmentally friendly PHB applications.