Preparation and Characterization of Papain Loaded Phosphatidyl Choline-PLGA Hybrid Nanoparticles as Novel Drug Delivery Systems

Polymeric nanoparticles possess the benefits of biocompatibility and stability, and also enable the controlled release of drugs. The matrix of the nanoparticle highlights the potential for an enhancement in the stability and efficacy of encapsulated therapeutic enzymes. The oral administration of lipid-polymer nanoparticle significantly improves mucus penetration, cellular uptake, and intracellular transport. The objective of this study is to develop a highly effective lipid-polymer nanoparticle system that is capable of absorbing Papain in the intestine. Papain loaded PLGA nanoparticles and Papain loaded PLGA-phosphatidylcholine nanoparticles were identified using FTIR analysis. Drug encapsulation efficiency of Papain loaded PLGA nanoparticles was found to be 49.20% and Papain loaded PLGA-Phosphatidylcholine nanoparticles was 77.5%. The drug loading capacity was found to be 3.75% and 6.84% for the Papain loaded PLGA- nanoparticles and Papain loaded PLGA-Phosphatidylcholine nanoparticles respectively. The antibacterial activity of Papain loaded PLGA-PC nanoparticles was found to be higher as compared to PLGA-PC nanoparticle for Staphylococcus aureus and Escherichia coli respectively. About 98% viability was observed in RAW 264.7 macrophage cells treated with the maximum concentration of 100 µg/ml of Papain-loaded PLGA-PC nanoparticles thereby depicting the biocompatibility property of the nanoparticles.