Green Synthesis of Carbohydrate Polymer Based Gum Kondagogu/Hydroxypropyl Cellulose Blend Silver Nanocomposite Film and Their Antimicrobial Activity

In the last few years, nanobiotechnology has undergone notable expansion, presenting a many applications driven by the practical viability of diverse synthesis techniques. The synthesis of silver nanocomposite through a green and eco-friendly approach is facilitated by polymer assistance, ensuring an efficient, cost-effective, and rapid process. This study specifically centers on creating eco-friendly and biodegradable nanocomposite films using hydroxypropyl (HP) cellulose and gum kondagogu (GK) through a simple casting process. In this method, silver nanocomposite (AgNPs) are synthesized by reducing AgNO₃, with the biopolymer gum kondagogu serving dual roles as both a reducing and stabilizing agent. The resulting composite and Ag nanocomposite films underwent thorough characterization using techniques such as X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy (FESEM & EDX), transmission electron microscopy, and X-ray photoelectron spectroscopy. XRD and FTIR results revealed robust interactions between AgNPs and the polysaccharide chains. TEM and SEM images demonstrated the uniformity spherical, and smooth surface of HPGKP-Ag nanocomposite films, with EDAX confirming the presence of elemental Ag in the film matrix. AFM images disclosed AgNPs dispersed on the surface of composite films. XPS data clarified that silver existed solely in metallic form, with migration occurring during film formation. Furthermore, antibacterial assessments against strains such as Pseudomonas, and Streptococcus exhibited notable growth inhibition for both strains, highlighting the significant antibacterial potential of these nanocomposite films.