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<p>The mammary gland is a dynamic organ whose parenchyma undergoes major development during puberty and extensive remodeling with each estrous cycle. These processes can be modelled and investigated in vitro via 3D cell culture techniques that employ specialized extracellular matrices and appropriate growth factors. The resulting mammary organoid cultures faithfully represent the mammary gland with respect to cellular heterogeneity, cell-cell contacts, overall architecture as well as response to growth factor stimuli and are amendable to a variety of molecular methods as well as microscopy techniques. Among the imaging techniques, light sheet microscopy (single plane illumination microscopy; SPIM) represents a useful method for longitudinal monitoring of morphological changes and cell behavior during the establishment of mammary gland ductal systems. In contrast to other fluorescence microscopy techniques such as widefield- and confocal-microscopy, SPIM exerts minimal phototoxicity while allowing fast acquisition of different fluorophores within organoids arranged in a 3D matrix under optimized environmental conditions. Here, we provide a detailed protocol for organoid acquisition and culture and describe two sample mounting variants for use with multiview and inverted light sheet microscopes.</p>

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Live-cell imaging of mammary organoids using light sheet microscopy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月1日

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  • Matea Brezak,
  • Azqa Ajmal Khan,
  • Martin Jechlinger,
  • Zuzana Sumbalova Koledova

摘要

The mammary gland is a dynamic organ whose parenchyma undergoes major development during puberty and extensive remodeling with each estrous cycle. These processes can be modelled and investigated in vitro via 3D cell culture techniques that employ specialized extracellular matrices and appropriate growth factors. The resulting mammary organoid cultures faithfully represent the mammary gland with respect to cellular heterogeneity, cell-cell contacts, overall architecture as well as response to growth factor stimuli and are amendable to a variety of molecular methods as well as microscopy techniques. Among the imaging techniques, light sheet microscopy (single plane illumination microscopy; SPIM) represents a useful method for longitudinal monitoring of morphological changes and cell behavior during the establishment of mammary gland ductal systems. In contrast to other fluorescence microscopy techniques such as widefield- and confocal-microscopy, SPIM exerts minimal phototoxicity while allowing fast acquisition of different fluorophores within organoids arranged in a 3D matrix under optimized environmental conditions. Here, we provide a detailed protocol for organoid acquisition and culture and describe two sample mounting variants for use with multiview and inverted light sheet microscopes.