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<p>The development of electrochemical sensors for γ-aminobutyric acid (GABA) remains challenging due to the limitations in sensitivity and selectivity encountered by many existing platforms. A nanoparticles-modified molecularly imprinted polymers (MIPs) sensor was successfully developed for the highly sensitive recognition and detection of GABA, a crucial biomarker associated with mental disorders. Notably, the synergistic combination of molybdenum disulfide nanoparticles (MoS<sub>2</sub> NPs) and gold nanoparticles (Au NPs) functioned as an efficient nanoparticle-mediated active layer, significantly enhancing the electrochemical response. Subsequently, a molecularly imprinted membrane was polymerized on this modified surface to achieve selective GABA recognition. To further improve detection performance, Prussian blue nanoparticles (PB NPs) were incorporated as a “built in” redox probe embedded into polypyrrole (PPY) framework. Additionally, a “Relative Oxidation Peak Height” analytical approach was introduced, effectively mitigating interference from various intrinsic and extrinsic factors. Under optimized conditions, the sensor demonstrated good linearity across a broad concentration range (1 nM−100 µM), where the reduction in redox probe current intensity correlated precisely with GABA concentration. The achieved limit of detection (LOD) was 7.82 × 10<sup>−10</sup> M (S/<i>N</i> = 3). Importantly, the sensor displayed minimal cross-reactivity toward common interfering neurotransmitters such as glutamic acid (Glu), serotonin (5-HT), Dopamine (DA) and acetylcholine (ACh). Validation experiments using artificial cerebrospinal fluid samples yielded recovery rates ranging from 96.65% to 105.2%, with relative standard deviation (RSD) values consistently below 5%. Our results reveal that the controllable preparation of high-quality GABA MIPs electrochemical sensor, or other neurotransmitter sensor, can use this preparation and testing methods.</p>

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Nanoparticle-Mediated Molecularly Imprinted Electrochemical Sensor MIPs/Au NPs/MoS2 NPs/SPE for Highly Sensitive Detection of γ-Aminobutyric Acid

  • 期刊论文

  • 发表日期：

    2025年12月23日

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  • Ping Sun,
  • Hongkai Fan,
  • Jia Han,
  • Xuman Ouyang,
  • Shenhui Ma,
  • Yuyu Bu,
  • Renxu Jia,
  • Xianying Dai

摘要

The development of electrochemical sensors for γ-aminobutyric acid (GABA) remains challenging due to the limitations in sensitivity and selectivity encountered by many existing platforms. A nanoparticles-modified molecularly imprinted polymers (MIPs) sensor was successfully developed for the highly sensitive recognition and detection of GABA, a crucial biomarker associated with mental disorders. Notably, the synergistic combination of molybdenum disulfide nanoparticles (MoS2 NPs) and gold nanoparticles (Au NPs) functioned as an efficient nanoparticle-mediated active layer, significantly enhancing the electrochemical response. Subsequently, a molecularly imprinted membrane was polymerized on this modified surface to achieve selective GABA recognition. To further improve detection performance, Prussian blue nanoparticles (PB NPs) were incorporated as a “built in” redox probe embedded into polypyrrole (PPY) framework. Additionally, a “Relative Oxidation Peak Height” analytical approach was introduced, effectively mitigating interference from various intrinsic and extrinsic factors. Under optimized conditions, the sensor demonstrated good linearity across a broad concentration range (1 nM−100 µM), where the reduction in redox probe current intensity correlated precisely with GABA concentration. The achieved limit of detection (LOD) was 7.82 × 10−10 M (S/N = 3). Importantly, the sensor displayed minimal cross-reactivity toward common interfering neurotransmitters such as glutamic acid (Glu), serotonin (5-HT), Dopamine (DA) and acetylcholine (ACh). Validation experiments using artificial cerebrospinal fluid samples yielded recovery rates ranging from 96.65% to 105.2%, with relative standard deviation (RSD) values consistently below 5%. Our results reveal that the controllable preparation of high-quality GABA MIPs electrochemical sensor, or other neurotransmitter sensor, can use this preparation and testing methods.