Tuning the Photoluminescence Properties of HAP-MWCNTs-CuO Composites Towards Sensitive Ochratoxin A Detection
摘要
In the present research, a sensitive and specific photoluminescence (Pl) based immunosensor was developed for the detection of ochratoxin A (OTA). The hydroxyapatite-multi-walled carbon nanotubes-copper oxide (Hap-MWCNTs-CuO) composite was deposited on glass substrate modified with aptes, and anti OTA (glass/Hap-MWCNTs-CuO-aptes-anti OTA). OTA was used to modify the structure, resulting in an OTA-selective layer. Real-time detection of OTA throughout a wide concentration spectrum from range 0–100 pgml−1 was made possible by the sensor's low detection limit of 0.11pgml−1. This study demonstrated the sensor's quick analytical capabilities across a variety of matrices and validated its excellent selectivity by successfully separating OTA from other analytes. The sensor provided real-time responses using four representative juice samples for examination, precisely detecting a range of OTA concentrations 1–50 ngml−1. In conclusion, the prepared biosensor provides a label-free, quick and sensitive approach for OTA detection, bringing new ideas for efficient mycotoxin surveillance in food safety. The development of a multiplex platform for the simultaneous monitoring of several fungal toxins is also made possible by this biosensor.