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<p>The improper disposal of laboratory waste poses tremendous environmental and health risks due to its hazardous chemical and biological content. The current study valorizes laboratory waste through an eco-friendly process for the biosynthesis of silver (Ag) and zinc oxide (ZnO) nanoparticles under different conditions. UV-vis spectroscopy, FTIR, XRD, and TEM techniques were used for nanoparticle characterization. Results revealed spherical Ag NPs (10–30&#xa0;nm) and ZnO NPs (30–50&#xa0;nm). Along with regression models, both nanoparticles demonstrated effective photocatalytic degradation of hazardous methylene blue (MB) and Congo red (CR) dyes under UV and daylight conditions, achieving degradation rates of 81% and 70%, respectively, within 60&#xa0;min. Additionally, NPs exhibited significant antimicrobial activity against various pathogens with MIC values ranging from 25 to 95&#xa0;µg/mL. Moreover, dose-dependent anticancer activity was observed against MCF-7 cells for both NPs, with IC<sub>50</sub> values ranging from 40 to 100&#xa0;µg/mL. Overall, the results show an innovative approach to reuse laboratory waste as a safe and green method for synthesizing NPs with promising applications in environmental remediation and medicinal drugs.</p>

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Unlock Laboratory Waste Potential to Turn into Sustainable Bi-metallic Nanoparticles with Dyes Degradation and Therapeutic Activities

  • 期刊论文

  • 发表日期：

    2025年3月21日

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  • Selma Hamimed,
  • Mohammed Zakaria Khelifati,
  • Rayene Hamimed,
  • Arbia Abbes

摘要

The improper disposal of laboratory waste poses tremendous environmental and health risks due to its hazardous chemical and biological content. The current study valorizes laboratory waste through an eco-friendly process for the biosynthesis of silver (Ag) and zinc oxide (ZnO) nanoparticles under different conditions. UV-vis spectroscopy, FTIR, XRD, and TEM techniques were used for nanoparticle characterization. Results revealed spherical Ag NPs (10–30 nm) and ZnO NPs (30–50 nm). Along with regression models, both nanoparticles demonstrated effective photocatalytic degradation of hazardous methylene blue (MB) and Congo red (CR) dyes under UV and daylight conditions, achieving degradation rates of 81% and 70%, respectively, within 60 min. Additionally, NPs exhibited significant antimicrobial activity against various pathogens with MIC values ranging from 25 to 95 µg/mL. Moreover, dose-dependent anticancer activity was observed against MCF-7 cells for both NPs, with IC50 values ranging from 40 to 100 µg/mL. Overall, the results show an innovative approach to reuse laboratory waste as a safe and green method for synthesizing NPs with promising applications in environmental remediation and medicinal drugs.