Multifunctional SnO₂-Chitosan-D-carvone Nanocomposite: A Promising Antimicrobial, Anticancer, and Antioxidant Agent for Biomedical Applications

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发表日期：

2024年11月5日

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Ghazi A. Bamagous,
Ibrahim Abdel Aziz Ibrahim,
Abdullah R. Alzahrani,
Imran Shahid,
Naiyer Shahzad,
Alaa H. Falemban,
Ibrahim Mufadhi M Alanazi,
Samer Hasan Hussein-Al-Ali,
Palanisamy Arulselvan,
Indumathi Thangavelu

摘要

Nanocomposite made up of inorganic and biocompatible polymer have gained significant attention for biomedical applications due to their enhanced multifunctional properties, offering solutions to serious issues like antimicrobial resistance and cancer treatment. Nanocomposite composed of SnO₂, chitosan and D-carvone (SnO₂-Cs-Dcar) was prepared to ascertain its efficacy in application for antimicrobial, anticancer activities, and antioxidant effects. The synthesized nanocomposite was characterized by XRD, UV-Vis, FTIR, PL, SEM, TEM, and XPS techniques, confirming successful integration. XRD results confirmed the tetragonal rutile phase of SnO₂. The band gap energy was calculated as 4.32 eV for SnO₂ nanoparticles and 3.11 eV for SnO₂-Cs-Dcar nanocomposite as observed from UV-Visible spectra. PL emission results showed that SnO₂-Cs-Dcar nanocomposite exhibited green emission at 507 nm corresponds to number oxygen vacancy site. SEM and TEM results showed that the SnO₂-Cs-Dcar nanocomposite entities appear more compact, and the single SnO₂ particles are less differentiated, possibly because they have been covered by chitosan and D-carvone. Antimicrobial activity against the pathogens Klebsiella pneumoniae, Candida albicans, Shigella dysenteriae, Bacillus subtilis, and Staphylococcus aureus demonstrated that SnO₂-Cs-Dcar exhibited enhanced bacteriostatic effect when compared to bare SnO₂. MTT assay on MOLT-4 cancer cells revealed that SnO₂-Cs-Dcar nanocomposite exhibited enhanced anticancer activity upon compared to SnO₂ nanoparticles. The IC₅₀ values were calculated as 13.6 for SnO₂ and 12.1 for SnO₂-Cs-Dcar nanocomposite. SnO₂-Cs-Dcar nanocomposites exhibits high antioxidant activity evidenced by improved free radical scavenging action in comparison with a bare SnO₂. Experimental result indicates that the SnO₂-Cs-Dcar nanocomposites can be used as biocidal agent for antimicrobial and anticancer therapies.