Innovative Nickel Oxide-Pluronic F127-Escin Hybrids: Synthesis, Characterization, and Dual Bioactivity Against Bacteria and Cancer Cells

The present work focused on synthesizing and characterizing nickel oxide-Pluronic127-Escin hybrid nanomaterials (NiO-PF127-Escin HNMs) and evaluating their antibacterial and cytotoxic properties against cervical (HeLa) and liver cancer (Hep3B) cells. The NiO-PF127-Escin HNMs were synthesized using a chemical precipitation process. XRD patterns revealed that the synthesized NiO-PF127-Escin HNMs exhibit a face-centered cubic structure with an average crystalline size of 42 nm. TEM analysis showed that the NiO-PF127-Escin HNMs formed a rod-like structure. The elemental composition of the hybrid materials, including Ni, O, and C, was identified by EDX spectrum analysis. DLS results indicated an average particle size of 145.90 nm, and UV–Vis spectral analysis revealed a maximum absorbance at 302 nm, confirming the formation of NiO-PF127-Escin HNMs. Various surface defects of the NiO-PF127-Escin HNMs were analyzed using PL. The NiO-PF127-Escin HNMs exhibited potential antibacterial activity against gram-negative bacterial strains. Additionally, NiO-PF127-Escin HNMs significantly inhibited the viability of both Hep3B and HeLa cells. The results suggest that NiO-PF127-Escin HNMs possess potent antibacterial and cytotoxic properties.