Utilization of Xanthan Gum-Silver Nitroprusside Nanoparticles for Prospective Advancements in Bacteriostasis and Wound Healing

Silver nitroprusside nanoparticles (AgNP NPs) offer valuable applications in both bacteriostasis and wound healing. Our research was the first to detail the methodology employed for the synthesis of novel nanomaterial Xanthan Gum-encapsulated AgNP NPs, yielding distinctive rod-like particles with an average size of 185.6 ± 0.45 nm. The synthesized XG-AgNP NPs demonstrated promising antibacterial and antibiofilm activities against various pathogens, including S. enterica, B. cereus, L. monocytogenes, E. coli, and S. aureus. The inhibitory effect of XG-AgNP NPs on S. enterica and S. aureus was attributed to the disruption of cell membranes, leading to protein and nucleic acid leakage. Furthermore, the cytotoxicity of XG-AgNP NPs against NIH3T3 cells was significantly lower compared to AgNP NPs. XG-AgNP NPs exhibited excellent biocompatibility and showed minimal hemolysis even at high concentrations (1 mg/mL). In vitro wound healing experiments, treatment with XG-AgNP NPs did not have a significant impact on NIH3T3 cells compared to the untreated control group.