<p>Prior research has established a positive association between signature strengths use and hedonic well-being. However, evidence concerning its relationship with eudaimonic well-being remains comparatively less explored. Moreover, the role of cultural factors in shaping these associations has received insufficient attention. This study explores how cultural context influences the association between signature strengths use and well-being, guided by the cultural fit hypothesis. Using data across 42 countries/regions and multilevel analyses, we examine whether signature strengths use benefits hedonic and eudaimonic well-being equally in individualistic and collectivistic cultures. Our findings show that signature strengths use positively predicts both hedonic and eudaimonic well-being. However, the strength of these associations varies markedly across cultural contexts. These associations are stronger in individualistic cultures and comparatively weaker in collectivistic cultures. These results suggest that cultural norms and values shape the extent to which signature strengths use contributes to well-being. By highlighting the moderating role of cultural context, this study underscores the need to consider cultural perspectives when designing and implementing positive psychology interventions. Integrating culturally sensitive approaches may enhance the effectiveness and relevance of strengths-based practices worldwide.</p>