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<p>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays a key role in diverse physiological and pathological processes, including immune responses, cancer progression and aging. Herein, we report a novel ratiometric fluorescent probe (CBN) for H<sub>2</sub>O<sub>2</sub> detection, synthesized via a one-step condensation reaction between 3-cyano-7-hydroxycoumarin and 4-(bromomethyl)benzeneboronic acid pinacol ester. The probe exhibited excellent selectivity and sensitivity toward H<sub>2</sub>O<sub>2</sub> (limit of detection [LOD] = 0.71 µM) with minimal interference from competing ions. Furthermore, CBN demonstrated application in exogenous H<sub>2</sub>O<sub>2</sub> detection in living HepG2 cells, highlighting its potential for biomedical research.</p>

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A Boronic Acid-Based Ratiometric Fluorescent Probe for Selective Detection of Hydrogen Peroxide

  • 期刊论文

  • 发表日期：

    2025年12月17日

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  • Ruilin Ge,
  • Ruijia Gan,
  • Rongrong Guo,
  • Xiaowei Xu,
  • Chaobing Gao

摘要

Hydrogen peroxide (H2O2) plays a key role in diverse physiological and pathological processes, including immune responses, cancer progression and aging. Herein, we report a novel ratiometric fluorescent probe (CBN) for H2O2 detection, synthesized via a one-step condensation reaction between 3-cyano-7-hydroxycoumarin and 4-(bromomethyl)benzeneboronic acid pinacol ester. The probe exhibited excellent selectivity and sensitivity toward H2O2 (limit of detection [LOD] = 0.71 µM) with minimal interference from competing ions. Furthermore, CBN demonstrated application in exogenous H2O2 detection in living HepG2 cells, highlighting its potential for biomedical research.