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<p>This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab<sub>1</sub>). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab<sub>2</sub>) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples.</p> Graphical Abstract <p></p>

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Magnetic MOF-Based Immunosensing Strategy with Quantum Dot Labeling for Efficient Electro-chemiluminescent Detection of Alpha-fetoprotein

  • 期刊论文

  • 发表日期：

    2025年11月3日

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  • Mariyam Fatima,
  • Muhammad Faizan,
  • Iqra Jabeen,
  • Muhammad Fiaz,
  • Ansar Ali,
  • Syed Kashif Ali,
  • Ali Bahadur,
  • Shahid Iqbal,
  • Sajid Mahmood,
  • Ibrahim Jafri,
  • Abd-ElAziem Farouk

摘要

This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab1). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab2) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples.

Graphical Abstract