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<p>Compared to monomer emission, excimer emission usually exhibits red-shifted, broadened, and large Stokes-shifted characteristics, which offer distinct advantages in biological imaging. In this work, we developed a rational design strategy for excimer-based cell membrane fluorescent probes, and on this basis, three fluorescent dyes (BPVP-C3, BPVP-C5 and BPVP-C8) exhibiting tunable excimer/monomer emission were obtained by covalent coupling of two pyrene units. In sodium dodecyl sulfate micelles, these fluorophores show bright excimer emission, whereas in dilute aqueous solutions, they emit monomer-dominated fluorescence. We proposed that the anionic micellar environment promotes intramolecular excimer formation between coupled pyrene units due to its balanced amphiphilicity, while these dye molecules can freely swing in dilute aqueous solution. Among them, BPVP-C8 exhibited optimal excimer-monomer switching property. Leveraging this environment-responsive dual-emission, it was explored as a wash-free cell membrane probe for simultaneous dual-channel (green and red) imaging.</p>

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Anionic Surfactant-Triggered Excimer Emission from Dipyrene-Based Amphiphilic Dyes for Wash-Free Cell Membrane Imaging

  • 期刊论文

  • 发表日期：

    2025年5月20日

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  • Yongling Zheng,
  • Bailin Guo,
  • Enju Wang,
  • Bingxin Lei

摘要

Compared to monomer emission, excimer emission usually exhibits red-shifted, broadened, and large Stokes-shifted characteristics, which offer distinct advantages in biological imaging. In this work, we developed a rational design strategy for excimer-based cell membrane fluorescent probes, and on this basis, three fluorescent dyes (BPVP-C3, BPVP-C5 and BPVP-C8) exhibiting tunable excimer/monomer emission were obtained by covalent coupling of two pyrene units. In sodium dodecyl sulfate micelles, these fluorophores show bright excimer emission, whereas in dilute aqueous solutions, they emit monomer-dominated fluorescence. We proposed that the anionic micellar environment promotes intramolecular excimer formation between coupled pyrene units due to its balanced amphiphilicity, while these dye molecules can freely swing in dilute aqueous solution. Among them, BPVP-C8 exhibited optimal excimer-monomer switching property. Leveraging this environment-responsive dual-emission, it was explored as a wash-free cell membrane probe for simultaneous dual-channel (green and red) imaging.