Polydimethylsiloxanes - Based Fluorescent Probe for H₂S Detection in Living Cells

The development of fluorescent probes for H₂S detection especially in living cells is of great significance due to its fundamental role as signal molecule. A promising scaffold for the development of such probes is polydimethylsiloxanes (PDMS), which is cost-effectiveness, non-toxicity, flexibility, and biocompatibility and easy to post-functionalize. Surprisingly, fluorescent probes for H₂S detection based on PDMS have not been investigated. Moreover, 4-nitro − 2,1,3-benzoxadiazole (NBD) derivates provides high fluorescence quantum yield, a large molar absorption coefficient, and sensitivity to environmental changes. Through reasonable design and adjustment of substituents on the NBD group, precise control of its fluorescence properties can be achieved. Herein, a novel H₂S fluorescent probe, P-NBD, was designed and synthesized by a one-step aromatic ring nucleophilic substitution of Cl-NBD with a PDMS derivative. Due to the occurrence of the cleavage reaction strategy and the intramolecular charge transfer process, P-NBD can detect H₂S via a colorimetric method. The limit of detection is down to 93 nM. P-NBD demonstrated considerable detection capability comparable to other reported types of H₂S probes. Moreover, the probe can also be utilized for H₂S imaging in HeLa cells. This work provides new insights into the design and synthesis of novel H₂S probes while also offering experimental evidence for the application of PDMS in cellular imaging.