Amino-Functionalized Metal–Organic Framework as Fluorescence Probe for Cell Imaging and Doxorubicin Detection

In this study, an NH₂–Cu–MOF was synthesized using the one-pot method and employed as a fluorescence probe for doxorubicin (DOX) detection. The synthesized NH₂–Cu–MOF exhibited remarkable fluorescence recognition capabilities for detecting DOX. Within the concentration range of 1–100 µmol/L, a linear relationship was observed between the fluorescence intensity of the NH₂–Cu–MOF and the DOX concentration. Furthermore, the synthesized NH₂–Cu–MOF was effectively utilized for highly selective fluorescence quenching recognition and quantitative detection of DOX in the presence of multiple metal ions and other antibiotics. Despite interference from multiple metal ions and antibiotics, DOX was identified and quantified by highly selective fluorescence quenching with a detection limit of 2.1654 µmol/L. These findings underscore the potential of NH₂–Cu–MOF as a class of “on–off” fluorescent probes for the rapid detection of DOX.