A New Phenothiazine-Based Fluorescent Probe for Rapid and Specific Detection of Fluoride

Fluorescent probes with specific and rapid response to fluoride ions are important mediators for detecting fluoride ions in biological systems. In this study, a phenothiazine-based fluorescent probe, PTC, was designed and synthesized, which undergoes cleavage activation and cyclization induced by fluoride ions targeting Si–O bonds. The probe exhibits strong anti-interference properties and reaches peak fluorescence within 5 min, allowing for quantitative detection of fluoride ions content in the concentration range of 0 to 12.5μM, suitable for live cell fluorescence imaging. The research findings suggest its potential application value in biological systems.