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<p>Phytophagous mites are known to possess olfactory sensitivity, enabling them to detect plant volatiles. Unlike insects, which detect plant volatiles primarily via odorant receptors (ORs), <i>Tetranychus cinnabarinus</i>, a highly destructive phytophagous species, lacks ORs and ionotropic receptors (IRs); it may rely on its retained ionotropic co-receptor (IR25a-like) genes for olfactory detection. Whether these IR25a-like receptor mediate plant volatile perception in this species remains unknown. Here, we cloned three IR25a-like genes—<i>TcinIR25a-1</i>, <i>TcinIR25a-2</i>, <i>and TcinIR25a-3</i>—and found that their expression was modulated by exposure to plant volatiles. RNA interference (RNAi) of <i>TcinIR25a-1</i> or <i>TcinIR25a-2</i> significantly altered female behavioral responses in Y-tube olfactometer assays, indicating their involvement in detecting plant volatiles. This study demonstrates for the first time that IR25a-like genes mediate plant volatile detection in <i>T. cinnabarinus</i>, offering new insights into mite olfactory mechanisms and potential molecular targets for behavior-based pest management.</p>

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IR25a-like Receptors are Required for Olfactory Detection of Plant Volatiles in the Carmine Spider Mite

  • 期刊论文

  • 发表日期：

    2026年3月10日

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  • Lishu Peng,
  • Qianqian Huang,
  • Wei Xiao

摘要

Phytophagous mites are known to possess olfactory sensitivity, enabling them to detect plant volatiles. Unlike insects, which detect plant volatiles primarily via odorant receptors (ORs), Tetranychus cinnabarinus, a highly destructive phytophagous species, lacks ORs and ionotropic receptors (IRs); it may rely on its retained ionotropic co-receptor (IR25a-like) genes for olfactory detection. Whether these IR25a-like receptor mediate plant volatile perception in this species remains unknown. Here, we cloned three IR25a-like genes—TcinIR25a-1, TcinIR25a-2, and TcinIR25a-3—and found that their expression was modulated by exposure to plant volatiles. RNA interference (RNAi) of TcinIR25a-1 or TcinIR25a-2 significantly altered female behavioral responses in Y-tube olfactometer assays, indicating their involvement in detecting plant volatiles. This study demonstrates for the first time that IR25a-like genes mediate plant volatile detection in T. cinnabarinus, offering new insights into mite olfactory mechanisms and potential molecular targets for behavior-based pest management.