<p>Parents of children with Autism Spectrum Disorder (ASD) experience higher levels of parenting stress than parents of neurotypical children or children with other conditions, with some evidence of the influence of certain child (e.g., externalizing behavior) and family (e.g., informant relationship to child) variables. Gaps in our understanding still exist in certain areas such as father’s parenting stress, stress experienced by parents who have multiple children diagnosed with ASD, and parent stress in diverse samples. This cross-sectional, retrospective study examined family and child variables as they relate to parenting stress in a large, diverse sample of parents at intake to early intensive behavioral intervention services. When examined in isolation, child variables such as challenging behavior, adaptive behavior, and age at diagnosis were differentially associated with parenting stress and one family variable, ethnicity of the family, was also differentially associated with parenting stress. When examined in a multiple regression analysis, the most influential variables were challenging behavior and family ethnicity.</p>