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<p>To address the limitations of traditional coating materials in eradicating various biofilms from surface, we formulated an amoxicillin-silver lipid nanoparticle (Ag-amox@LNP). Silver nanoparticles (AgNPs) were obtained from the leaves of <i>Schleichera oleosa</i> (Kusum), a native plant of Jharkhand, coat them with lipids to mitigate oxidation, and evaluate their efficacy in inhibiting biofilm formation. The novelty of this work lies in the green synthesis of silver nanoparticles using <i>Schleichera oleosa</i> plant extract, which has been utilized for the first time as a reducing agent for silver nitrate. This approach introduces unique bioactive properties from the plant extract. Silver nanoparticles were subsequently modified with amoxicillin and lipid, enhancing their antimicrobial efficacy approximately 2.9 ± 0.6&#xa0;mm zone of inhibition was observed, reducing the oxidation of silver and amoxicillin, while minimizing aggregation. Ag-amox@LNP were employed to target the extracellular polymeric substance (EPS) matrix of methicillin resistant <i>S. aureus</i>. The nanoparticles were characterized using field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), confocal laser fluorescence microscopy (CFLM), and UV spectroscopy. The results demonstrated particle size (Z-average) of Ag-NP and Ag-amox@LNP were 56 ± 9&#xa0;nm and 344 ± 51&#xa0;nm respectively, they showed low cytotoxicity, and superior specificity towards MR <i>S. aureus</i> compared to free amoxicillin and non-targeting controls. The results showed that these nanoparticles were effective in eradicating MR <i>S. aureus</i> planktonic cells at a concentration of 0.5&#xa0;mg/mL, while that in reducing biofilm and embedded cell at min concentration 1&#xa0;mg/ml. In vitro studies revealed enhanced antibiofilm activity.</p> Graphical Abstract <p></p>

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Novel UV-facilitated Green Synthesis of Silver Nanoparticles with Schleichera Oleosa Leaf Extract and its Functionalisation on Lipid Nanoparticles for amoxicillin-silver Conjugated Delivery System against Methicillin Resistant S.aureus Biofilm Reduction

  • 期刊论文

  • 发表日期：

    2025年11月12日

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  • Kajal Kumari,
  • Hare Ram Singh,
  • Muthu Kumar Sampath

摘要

To address the limitations of traditional coating materials in eradicating various biofilms from surface, we formulated an amoxicillin-silver lipid nanoparticle (Ag-amox@LNP). Silver nanoparticles (AgNPs) were obtained from the leaves of Schleichera oleosa (Kusum), a native plant of Jharkhand, coat them with lipids to mitigate oxidation, and evaluate their efficacy in inhibiting biofilm formation. The novelty of this work lies in the green synthesis of silver nanoparticles using Schleichera oleosa plant extract, which has been utilized for the first time as a reducing agent for silver nitrate. This approach introduces unique bioactive properties from the plant extract. Silver nanoparticles were subsequently modified with amoxicillin and lipid, enhancing their antimicrobial efficacy approximately 2.9 ± 0.6 mm zone of inhibition was observed, reducing the oxidation of silver and amoxicillin, while minimizing aggregation. Ag-amox@LNP were employed to target the extracellular polymeric substance (EPS) matrix of methicillin resistant S. aureus. The nanoparticles were characterized using field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), confocal laser fluorescence microscopy (CFLM), and UV spectroscopy. The results demonstrated particle size (Z-average) of Ag-NP and Ag-amox@LNP were 56 ± 9 nm and 344 ± 51 nm respectively, they showed low cytotoxicity, and superior specificity towards MR S. aureus compared to free amoxicillin and non-targeting controls. The results showed that these nanoparticles were effective in eradicating MR S. aureus planktonic cells at a concentration of 0.5 mg/mL, while that in reducing biofilm and embedded cell at min concentration 1 mg/ml. In vitro studies revealed enhanced antibiofilm activity.

Graphical Abstract