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<p>The green synthesis of metal nanoparticles using plant-based molecules is gaining attention for its potential in biomedical applications. The aim of present study the eco-friendly synthesis of gold nanoparticles (AuNPs) using ellagic acid, a natural antioxidant, which acted as both reducing and capping agent. The successful synthesized ellagic acid-coated AuNPs (EA-AuNPs) was indicated by a colour change to dark brown and confirmed through UV-visible spectroscopy with a peak at 587.5&#xa0;nm. Transmission electron microscopy (TEM) revealed long rod-shaped nanoparticles ranging from 50 to 70&#xa0;nm in size, and energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of Au, C, Cl, and N elements. Zeta potential analysis showed a stable surface charge of -17.7 mV. EA-AuNPs exhibited strong antibacterial activity, including a 37.26 ± 0.9&#xa0;mm inhibition zone against <i>Klebsiella pneumoniae</i> at 100&#xa0;µg/mL and minimum inhibitory concentration (MIC) values below 1&#xa0;µg/mL against <i>Staphylococcus aureus</i>. The nanoparticles also demonstrated potent antioxidant activity, achieving 91% and 89% radical scavenging in DPPH and ABTS assays, respectively. Anti-inflammatory testing showed 78% membrane stabilization at 200&#xa0;µg/mL. Moreover, cytotoxicity studies revealed that EA-AuNPs had an IC₅₀ of 59.58&#xa0;µg/mL against oral epidermoid carcinoma (KB) cells. In-silico analysis also demonstrated a promising binding affinity of -7.3&#xa0;kcal/mol with <i>Candida albicans</i>, suggesting notable antifungal properties. The present study highlights the multi-functional therapeutic potential of EA-AuNPs in oral health care, offering a biocompatible approach to combat microbial infections, oxidative stress, inflammation, and oral cancer. The integration of both in-vitro and in-silico results supports their future application in dental and oral medicine.</p>

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Ellagic Acid-Wrapped Gold Nanoparticles: Evaluation of Antimicrobial, Antioxidant, Anti-Inflammatory, and Cytotoxicity Effects of KB Cells

  • 期刊论文

  • 发表日期：

    2025年6月2日

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  • Muskan Soni,
  • Chinnasamy Ragavendran,
  • Mohamed Imath,
  • Salim Manoharadas,
  • Ismini Nakouti

摘要

The green synthesis of metal nanoparticles using plant-based molecules is gaining attention for its potential in biomedical applications. The aim of present study the eco-friendly synthesis of gold nanoparticles (AuNPs) using ellagic acid, a natural antioxidant, which acted as both reducing and capping agent. The successful synthesized ellagic acid-coated AuNPs (EA-AuNPs) was indicated by a colour change to dark brown and confirmed through UV-visible spectroscopy with a peak at 587.5 nm. Transmission electron microscopy (TEM) revealed long rod-shaped nanoparticles ranging from 50 to 70 nm in size, and energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of Au, C, Cl, and N elements. Zeta potential analysis showed a stable surface charge of -17.7 mV. EA-AuNPs exhibited strong antibacterial activity, including a 37.26 ± 0.9 mm inhibition zone against Klebsiella pneumoniae at 100 µg/mL and minimum inhibitory concentration (MIC) values below 1 µg/mL against Staphylococcus aureus. The nanoparticles also demonstrated potent antioxidant activity, achieving 91% and 89% radical scavenging in DPPH and ABTS assays, respectively. Anti-inflammatory testing showed 78% membrane stabilization at 200 µg/mL. Moreover, cytotoxicity studies revealed that EA-AuNPs had an IC₅₀ of 59.58 µg/mL against oral epidermoid carcinoma (KB) cells. In-silico analysis also demonstrated a promising binding affinity of -7.3 kcal/mol with Candida albicans, suggesting notable antifungal properties. The present study highlights the multi-functional therapeutic potential of EA-AuNPs in oral health care, offering a biocompatible approach to combat microbial infections, oxidative stress, inflammation, and oral cancer. The integration of both in-vitro and in-silico results supports their future application in dental and oral medicine.