Enhanced Wound Healing Activity in Animal Model via Developing and Designing of Self-nano Emulsifying Drug Delivery System (SNEDDS) for the Co-delivery of Hesperidin and Rutin

The aim of this work is to develop a self-nanoemulsifying drug delivery system (SNEDDS) for Hesperidin (HES) and Rutin (RUT) to improve their biopharmaceutical properties. The wound healing potential of HES-RUT-SNEDDS was compared to those of pure HES suspension (HES-s), empty SNEDDS (E-SNEDDS), and standard Fusidic Acid via topical application. To produce various HES-RUT-loaded SNEDDS, aqueous phase titration was used to select cinnamon oil, Labrasol and Tween 80 (surfactants), Transcutol (co-surfactant) from a diverse pool of surfactants, oils and co-surfactants. The thermodynamic stability of HES-RUT-loaded SNEDDS was assessed by examining the globule size, surface morphology, zeta potential, polydispersity index (PDI), and percent (%) transmittance. The improved physicochemical properties of the optimized HES-RUT-SNEDDS (S-N4) formulation included particle size, zeta potential, and % transmittance. Smooth and spherical particles were discovered using Atomic Force Microscopy (AFM). These improved SNEDDS formulations demonstrated enhanced solubility and skin permeation. When compared to HES-s, E-SNEDDS, and standard fusidic acid, the optimized HES-RUT-SNEDDS demonstrated significant wound healing activity following topical application. HES-RUT-SNEDDS is a promising approach for enhancing the wound-healing potential of HES and RUT through topical administration.