Synthesis of Bimetallic Copper oxide-silver Nanoparticles by Gum Arabic: Unveiling Antimicrobial, and Antibiofilm Potential against some Pathogenic Microbes Causing Wound Infection

The adoption of environmentally friendly approaches in NPs production represents a pivotal stride towards achieving sustainability in nanotechnology through biomass conversion. Due to their potential for synergy at low concentrations and biological applications, bimetallic nanoparticles have drawn a lot of attention recently. In the current work, Gum Arabic as a biological macromolecules source was used to create bimetallic copper oxide-silver nanoparticles (CuO-Ag NPs) in an economical and environmentally friendly manner. Bimetallic CuO-Ag NPs were successfully synthesized at the nanoscale, showed excellent dispersion, and formed stable colloidal nano-solutions, according to their characterization. Additionally, the antifungal and antibacterial potentials of bimetallic CuO-Ag NPs were assessed in relation to some pathogenic microbes that were isolated from the sides of wounds. Also, growth curve assay had been performed to P. aeruginosa, and S. epidermidis after the treatment by bimetallic CuO-Ag NPs. Finally, antibacterial reaction mechanism had been determined by SEM imaging process. Bimetallic CuO-Ag NPs’ minimum inhibitory concentration (MIC) was determined for every tested strain of yeast and bacteria. With inhibition zones of 22 ± 1.80 mm, and 26 ± 1.0 mm (at a concentration of 50 µg/mL), the results showed the antibacterial activity of bimetallic CuO-Ag NPs against P. aeruginosa, and S. epidermis, respectively. In summary, Gum Arabic was effectively used to create bimetallic CuO-ZnO NPs, which demonstrated promising antimicrobial properties, paving the path for their safe implementation in a variety of biomedical applications specially to treat some pathogenic microbes-causing wound infection.