Optimized Nanoscale Mineralization Strategy for Maintaining Bio-enzymatic Stability

Conventional biomineralization strategies are constructed using microparticles, suffering the inhibition of protease bio-availability and required rigorous bio-evaluation. The use of nanomineralization technology could apply control over the size, shape, and composition of the materials and provide a steadier maintenance environment for the bio-enzyme. In this study, stable and nanoscale copper phosphate (Cu₃(PO₄)₂) bio-nanomineralization materials were constructed to encapsulate free bio-enzyme. By optimizing the biomineralization process, Cu₃(PO₄)₂ was demonstrated to enable the facile coordination of various bio-enzymes (e.g., catalase or β-glucosidase) for efficient bio-enzyme transportation and maintaining the bio-enzyme activity in harsh condition. The current nanomineralization strategy could provide new ideas and methods for the development of biocatalysis, biosensing, biomedicine, and other related fields.