Inhibition of Virulence Properties of Pathogenic Pseudomonas aeruginosa by Rutin-Loaded Chitosan Nanoparticles

Due to biofilm formation and the development of antibiotic resistance, alternative approaches are required to counter Pseudomonas aeruginosa infections. Additionally, P. aeruginosa produces several virulence factors that play a crucial role in chronic infections. The current study aimed to synthesize rutin-loaded chitosan nanoparticles (RUT-CS NPs) and investigate their antibacterial potential against pathogenic P. aeruginosa strains. RUT-CS NPs were synthesized through ionic gelation technique and characterized using FTIR, XRD, DLS, Zeta, SEM, and TEM analyses. The characterization of the synthesized NPs revealed an amorphous structure with a diameter ranging from 230 to 280 nm. The motility of P. aeruginosa, including swarming, swimming, and twitching, was notably diminished by RUT-CS NPs. Furthermore, the NPs exhibited a substantial biofilm inhibitory effect on P. aeruginosa. Moreover, RUT-CS NPs decreased bacterial pyocyanin levels and proteolytic activity. Findings from the current research demonstrate the potential of RUT-CS NPs as a promising novel treatment option for managing P. aeruginosa infections.