<p>In this work, an efficient magnesium-doped titanium oxide (Mg@TiO₂)-based electrochemical sensor was developed for the detection of glucose under alkaline conditions. The developed non-enzymatic glucose sensor demonstrated excellent analytical performance with a simple preparation method. The synergistic interactions between magnesium (Mg) dopants and titanium dioxide promote rapid electron transfer, better catalytic behavior, and absorption of glucose on the electrode surface. The incorporation of the Mg@TiO₂ nanocomposite was confirmed by X-ray diffractometer (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. The glucose sensing behavior of the fabricated electrode was analyzed using cyclic voltammetry (CV) and amperometry methods. The interfacial charge-transfer properties of the Mg@TiO₂ nanocomposite were investigated by electrochemical impedance spectroscopy (EIS). The prepared Mg@TiO₂ electrode exhibited high sensitivity of 2.71 µA mM⁻¹&#xa0;cm⁻², across a wide linear range with a low detection limit of 20&#xa0;µM. The fabricated electrode exhibited superior selectivity and long-term stability, showing negligible interference effects. The defect-induced Mg@TiO₂-fabricated electrode, promoted as a highly active and stable redox material, suggests enhanced electrochemical response. In addition, the fabricated electrode was tested in human blood serum to confirm its real-time applicability, highlighting its potential as a promising non-enzymatic sensor for accurate blood glucose detection.</p>