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<p>Dengue fever affects millions globally, necessitating early diagnosis to prevent severe complications such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). This study presents the development of a smartphone-based portable immunosensor for the stable and rapid detection of the Dengue virus non-structural protein 1 (DENV-NS1) antigen. The immunosensor utilizes a screen-printed carbon electrode (SPCE) enhanced with a polyvinyl alcohol (PVA) coating to improve stability and performance. Field emission scanning electron microscopy (FESEM) is used to observe the changes in surface morphology of the working electrode. The EDC/NHS carbodiimide chemistry is employed to immobilize antibodies on the SPCE surface, ensuring precise detection of dengue NS1 along with improved sensitivity. Integrated with the portable "Sensit" electrochemical device, this biosensor achieves a low limit of detection (LOD) of 22.43&#xa0;ng/mL and demonstrates high specificity with minimal nonspecific binding against other viral NS1 proteins. The PVA coating ensures excellent stability, allowing for reliable storage for up to 9&#xa0;weeks at 4&#xa0;°C. The immunosensor offers a fast response, minimal sample preparation, and easy operation via a smartphone interface. Clinical validation underscores its effectiveness, making it a promising tool for point-of-care dengue virus detection, offering accuracy and sensitivity comparable to laboratory-based sensors.</p>

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Development of a smartphone-based portable immunosensor with PVA coating for stable detection of dengue virus

  • 期刊论文

  • 发表日期：

    2025年5月24日

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  • Aishwarya Kapse,
  • Sohel Shaikh,
  • Arpita Pandey-Tiwari

摘要

Dengue fever affects millions globally, necessitating early diagnosis to prevent severe complications such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). This study presents the development of a smartphone-based portable immunosensor for the stable and rapid detection of the Dengue virus non-structural protein 1 (DENV-NS1) antigen. The immunosensor utilizes a screen-printed carbon electrode (SPCE) enhanced with a polyvinyl alcohol (PVA) coating to improve stability and performance. Field emission scanning electron microscopy (FESEM) is used to observe the changes in surface morphology of the working electrode. The EDC/NHS carbodiimide chemistry is employed to immobilize antibodies on the SPCE surface, ensuring precise detection of dengue NS1 along with improved sensitivity. Integrated with the portable "Sensit" electrochemical device, this biosensor achieves a low limit of detection (LOD) of 22.43 ng/mL and demonstrates high specificity with minimal nonspecific binding against other viral NS1 proteins. The PVA coating ensures excellent stability, allowing for reliable storage for up to 9 weeks at 4 °C. The immunosensor offers a fast response, minimal sample preparation, and easy operation via a smartphone interface. Clinical validation underscores its effectiveness, making it a promising tool for point-of-care dengue virus detection, offering accuracy and sensitivity comparable to laboratory-based sensors.