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<p>In this study, we developed a highly sensitive and selective fluorescence nanoprobe based on zinc and silver-modified carbon quantum dots (CQDs/Ag/Zn) for the detection of tetracycline. The CQDs/Ag/Zn were synthesized through a hydrothermal method, utilizing zinc nitrate, silver nitrate, and Artemisia absinthium as a carbon source. Carbon quantum dots are gaining significant attention as fluorescent nanomaterials due to their remarkable sensitivity and selectivity in detecting biological molecules. In our investigation, we observed a notable increase in the fluorescence signal of CQDs/Ag/Zn specifically in the presence of tetracycline. This characteristic enables effective detection of this widely used antibiotic, making the capability to identify it in biological samples of utmost importance. The fluorescence signal was found to increase prominently at a pH of 6 in the presence of tetracycline while effectively avoiding interference from other substances. After optimizing various parameters influencing the sensor’s response, we established a linear detection range from 16.6 to 233.33&#xa0;<i>µ</i>M, with a detection limit of 6.5&#xa0;<i>µ</i>M. Furthermore, the capability of the sensor for practical application was assessed through the determination of tetracycline levels in human blood plasma samples. The results obtained demonstrate the potential of this nanoprobe for use in clinical applications and toxicological studies.</p>

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Determination of tetracycline in milk samples using CQDs/Ag/Zn fluorescent nanoprobe

  • 期刊论文

  • 发表日期：

    2025年6月4日

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  • Sara Bijari,
  • Bahareh Rahimian Zarif

摘要

In this study, we developed a highly sensitive and selective fluorescence nanoprobe based on zinc and silver-modified carbon quantum dots (CQDs/Ag/Zn) for the detection of tetracycline. The CQDs/Ag/Zn were synthesized through a hydrothermal method, utilizing zinc nitrate, silver nitrate, and Artemisia absinthium as a carbon source. Carbon quantum dots are gaining significant attention as fluorescent nanomaterials due to their remarkable sensitivity and selectivity in detecting biological molecules. In our investigation, we observed a notable increase in the fluorescence signal of CQDs/Ag/Zn specifically in the presence of tetracycline. This characteristic enables effective detection of this widely used antibiotic, making the capability to identify it in biological samples of utmost importance. The fluorescence signal was found to increase prominently at a pH of 6 in the presence of tetracycline while effectively avoiding interference from other substances. After optimizing various parameters influencing the sensor’s response, we established a linear detection range from 16.6 to 233.33 µM, with a detection limit of 6.5 µM. Furthermore, the capability of the sensor for practical application was assessed through the determination of tetracycline levels in human blood plasma samples. The results obtained demonstrate the potential of this nanoprobe for use in clinical applications and toxicological studies.