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<p>We present a sensitive colorimetric sensor based on platinum (Pt) nanoparticle-decorated graphitic carbon nitride (Pt@g-C<sub>3</sub>N<sub>4</sub>) for the detection of ascorbic acid (AsA) in real samples. Pt@g-C<sub>3</sub>N<sub>4</sub> nanocomposites were synthesized through surface reduction of Pt ions on g-C<sub>3</sub>N<sub>4</sub> dispersions, with their synthesis confirmed by various characterization techniques. The nanocomposites exhibited enhanced oxidase-like activity, particularly in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Utilizing AsA’s competitive inhibition of the oxidase activity, we developed a colorimetric assay for quantifying AsA in acetate buffer, commercially available tablets, and real samples such as orange and lemon juice. The sensor demonstrated a linear response in the 1–100&#xa0;μM range, with a limit of detection of 0.28&#xa0;μM. Additionally, we introduced a paper-based assay for on-site, naked-eye detection of AsA. This work offers a practical and cost-effective approach for monitoring AsA levels in various food and pharmaceutical products, with potential applications in health diagnostics and quality control.</p>

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Enhanced oxidase activity of platinum decorated graphitic carbon nitride for the colorimetric detection of ascorbic acid

  • 期刊论文

  • 发表日期：

    2025年4月21日

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  • Priya Singh,
  • Ravi Prakash Ojha,
  • Subhajit Jana,
  • Aniruddha Jaiswal,
  • Rajiv Prakash

摘要

We present a sensitive colorimetric sensor based on platinum (Pt) nanoparticle-decorated graphitic carbon nitride (Pt@g-C3N4) for the detection of ascorbic acid (AsA) in real samples. Pt@g-C3N4 nanocomposites were synthesized through surface reduction of Pt ions on g-C3N4 dispersions, with their synthesis confirmed by various characterization techniques. The nanocomposites exhibited enhanced oxidase-like activity, particularly in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Utilizing AsA’s competitive inhibition of the oxidase activity, we developed a colorimetric assay for quantifying AsA in acetate buffer, commercially available tablets, and real samples such as orange and lemon juice. The sensor demonstrated a linear response in the 1–100 μM range, with a limit of detection of 0.28 μM. Additionally, we introduced a paper-based assay for on-site, naked-eye detection of AsA. This work offers a practical and cost-effective approach for monitoring AsA levels in various food and pharmaceutical products, with potential applications in health diagnostics and quality control.