Application of molecularly imprinted polymer as an efficient media for the design of glibenclamide selective sensor: computational studies and electrochemical determination

In this project, the imprinted polymer glibenclamide (GLB) was synthesized by electropolymerization of pyrrole (PY) on a pencil graphite electrode (PGE). Calculation methods was used for choosing the PY as the suitable monomer. To obtain optimal conditions, the experiments were designed, in two different steps, according Plackett–Burman design (PBD) and central composite design (CCD) techniques. Synthesis of the sensor and all measurements were performed via cyclic (CV) and differential pulse voltammetry (DPV), respectively. Field emission scanning electron microscope (FE-SEM) was utilized for investigation of the surface structures of the fabricated sensor. Linear detection range from 0.1 to 1000 μM with limits of detection and quantification of 0.05 μM and 0.17 μM were obtained in optimal conditions. Moreover, the synthesized sensor showed acceptable repeatability (RSD, 3%), reproducibility (RSD, 8.6%), and stability for up to one month (recovery, 91%). The sensor was used for extraction and determination of GLB in the presence of some medicine as interferents, successfully. The synthesized MIP-PGE film showed a good electrochemical response to monitor GLB in real samples of tablet and human serum.